摘要
根据在醋酸-醋酸钠缓冲介质中，痕量镍离子对过氧化氢氧化丁基罗丹明B（BRB）褪色反应有催化作用原理，建立了测定卷烟中痕量镍的催化光度法。即将烟丝样品消化液与2．5mL pH5．0的醋酸-醋酸钠缓冲溶液、2．0mL 2％TritonX-100溶液、3．5mL 3％过氧化氢溶液和3．0mL0．01％BRB溶液，在75℃水浴中反应8min，于560nm波长下检测其吸光度。在0.32～12．0μg／L线性浓度范围内，方法检出限为0．078μg／L，RSD为2．2％～4．2％，回收率为97．3％～102．7％。该法的测定结果与原子吸收光谱法的吻合。
A method for determining trace nickel in cigarette by catalytic kinetic spectrophotometry was developed based on that Ni （ Ⅱ ） has a catalytic effect on the oxidative discoloration of butylrhodamine - B （BRB） with hydrogen peroxide in HAc - NaAc buffer solution. 2.0 mL digested tobacco solution was reacted with 2.0 mL 2% TritonX - 100, 3.5 mL 3% hydrogen peroxide, and 3.0 mL 0.01% BRB in the presence of 2.5 mL pH5.0 HAc - NaAc buffer solution for 8 minutes in a water bath at 75℃ and then detected at the wavelength of 560 nm. The recovery of Ni （ Ⅱ ） was from 97.3% to 102.7% with RSD of 2.2% to 4.2% , the limit of detection of 0. 078 μg/L in the linear concentration range of 0.32 to 12.0 μg/L. The Ni （ Ⅱ ） content in tobacco determined with this method agreed with the results of atomic absorption speetrophotometry.
出处
《烟草科技》
EI
CAS
北大核心
2009年第1期50-53,共4页
Tobacco Science & Technology
基金
郧阳师范高等专科学校校立科研项目"新型动力学反应体系测定过渡金属的研究"(2006C01)
关键词
催化光度法
镍
卷烟
丁基罗丹明B
Catalytic kinetic spectrophotometry
Nickel
Cigarette
Butylrhodamine - B
