摘要
以一粒小麦-葡萄牙野燕麦杂交后代一粒葡为实验材料,以地高辛标记的葡萄牙野燕麦基因组DNA为探针、一粒小麦基因组DNA为封阻对一粒葡及其衍生系根尖染色体进行基因组原位杂交(GISH)分析,探讨了影响一粒葡GISH效果的主要因素.建立并优化了一粒葡GISH分析的实验体系,即探针DNA与封阻DNA比例为1∶50时可有效分开双方染色体组.优化GISH分析显示,在一粒葡后代衍生系中均检测到燕麦染色质的存在,且不同选系间带有燕麦染色体的数目不同,进一步证明一粒葡是一粒小麦-葡萄牙野燕麦远缘杂交的后代.
We used ＇YLP＇,the distant hybridization generations from Avena fatua and Triticum monococcum as experiment materials.GISH with genomic DNA of A.fatua labeled by DIG as probe and genomic DNA of T.monococcum as blocker was used to identify alien chromosome in root tip chromosomes of ＇YLP＇,to analyze the main factors influencing GISH results,to establishe optimal GISH system.The GISH result showed that the chromosomes of A.fatua could be detected from T.monococcum at the concentration ratio on 1∶50 of probe DNA and blocker DNA,and different lines showed different numbers of A.fatua,and verified ＇YLP＇ were the distant hybridization generations from A.fatua and T.monococcum.
出处
《西北植物学报》
CAS
CSCD
北大核心
2008年第12期2442-2446,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家重点基础研究发展规划项目(2006CB100203)
教育部长江学者和创新团队发展计划项目(IRT0558)
国家自然基金项目(30871603)
