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紫外分光光度法测定长白楤木根、茎、叶中总黄酮含量 被引量:7

Determination of the Total Flavonoids in Roots,Stalks and Leaves of Aralia continentalis Kitagwa by Spectrophotometry
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摘要 目的对长白楤木根、茎、叶中所含总黄酮的含量进行测定。方法采用紫外分光光度法于360nm波长处测定。结果总黄酮在4.08~32.64μg/ml范围内线性关系良好,其回归方程为:A=0.0183C+0.0127,R^2=0.9992;平均加样回收率为100.63%(RSD=1.93%)。结论该方法简便、准确,可用于长白楤木的质量控制。 Objective To establish a method for the determination of the total flavonoids in roots, stalks and leaves of Aralia con- tinentalis Kitagwa. Methods The total flavonoids in roots, stalks and leaves of Aralia continentalis Kitagwa were determined by spectrophotometric method at 360 nm wavelength. Results Sample size showed a good linear relationship at the range of 4.08 ~32.64 μg/ml,and the curve was A =0.0183C +0. 012 7, R2 =0. 999 2. The average recovery was 96.2% and RSD was 1.42% (n = 6 ). Conclusion This method is easy and accurate, and can be used for quality control of Aralia continentalis Kitagwa.
出处 《时珍国医国药》 CAS CSCD 北大核心 2009年第1期55-56,共2页 Lishizhen Medicine and Materia Medica Research
基金 黑龙江省农垦总局项目(No.HNKXIV-06-06-04)
关键词 长白楤木 紫外分光光度法 总黄酮 Aralia continentalis Kitagwa Spectrophotometric method Total flavonoids
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