摘要
为了观察单纯疱疹病毒（ＨＳＶ）对脑膜和脑实质的侵袭性，用不同剂量的ＨＳＶ体外感染兔脑膜及脑神经细胞，对病毒的感染强度作趋势检验，同时观察该病毒的致细胞病变作用及抗原分布。ＨＳＶ对体外兔脑膜和脑神经细胞均造成明显的病理损害，趋势分析Ｐ＞０．０５，两种细胞的胞浆与胞核中均有病毒抗原分布。
This experiment is to compare HSV 1 invasiveness in two different tissues,meningocyte and cerebral nerve cells in vitro.The cells were from New Zealand rabbits and taken primary cell culture.The two kinds of cells were infected with HSV 1 in various dosages when cells were cultured 5 to 7 days.Cell pathologic effect(CPE) was observed every 12h after infection and scored on the 7th day for trend analysis.The results showed HSV 1 can cause visible pathologic changes in both kinds of cells.The cytopathic effect was characterized by swelling,aggregation and fusion with cell lysis and death.Trend analysis showed P >0.05,which suggests HSV 1 invasiveness was of no significant difference in the two kinds of cell in vitro.Indirect immunofluorescence staining with human antiserum to HSV 1 was positive in the pathologic cells.
出处
《山东医科大学学报》
1998年第2期131-134,共4页
Acta Academiae Medicinae Shandong
关键词
人疱疹病毒
脑膜
脑实质
病毒性脑炎
Herpesvirus hominis
Invasiveness
Meninges
Neurons
In vitro
Rabbits
