摘要
目的对浙江新分离的汉坦病毒疫苗后备筛选毒株ZJ5株的M和S全片段进行基因序列测定与基因分型，了解该毒株分子基础，并分析与其他病毒株以及20年前浙江分离的汉坦病毒Z10、Z37疫苗株的差异。方法提取汉坦病毒ZJ5株感染细胞总RNA，进行逆转录PCR扩增，产物纯化后克隆于T载体并测定序列。结果扩增出ZJ5病毒M和S全片段并测定了序列，经对核苷酸和氨基酸序列分析表明，与汉城型（SEO）病毒有最高的同源性，为SEOV，但与国内外已知的SEOV核苷酸差异高达11．7％～19．2％和6．7％～14．5％；用M和S片段核苷酸序列所构建的系统进化树显示，病毒株分在SEOV发生群，与SEOV的Gou3株亲缘关系最近，但独自构成一进化支。结论ZJ5株为一不同于现已报道的SEO型毒株的新亚型．
Objective In order to understand the molecular characters of Hantavirus ZJ5 strain, its complete M and S genome were sequenced and compared with that of other hantavirus strains. Methods We prepared the total RNA from ZJ5. Infected ceils and the raw or purified RT-PCR product was cloned and sequenced. Results With sequence compation, we found ZJ5 strain complete M and S segment had higher homology with SEO-type strains than other type of HV, but differential genes were 11.7%-19.2% and 6.7%- 14.5 % from SEOV. The phylogenetic trees constructed by complete M and S segment showed that ZJ5 strain was located in SEOV group, and structured alone embranchment. Conclusion The ZJ5 strain was believed to belong to SEO-type virus, and suggest that ZJ5 strain is a new subtype S SEOV group, and structured alone embranchment. Conclusion The ZJ5 strain was beliceved to belong to SEO-type virus, and suggest that ZJ5 strain is a new subtype from other SEO viruses.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2008年第6期434-436,共3页
Chinese Journal of Experimental and Clinical Virology
基金
国家“863”科技攻关项目（2007AA02Z465）
国家自然科学基金（30771861）
浙江省自然科学基金资助（300493）
关键词
汉坦病毒
序列测定
系统发生树
Hantavirus
Sequence analysis
Phylogenetic tree
