摘要
目的:探讨潜活膜蛋白1(latent membrane protein 1,LMP-1)、NET-1、血管内皮生长因子(vascular endothelial growth factor,VEGF)在非角化性鼻咽癌(non-keratin nasopharyngeal carcinoma,NK-NPC)中的表达及其临床意义。方法:取南通大学附属医院1999年5月-2003年5月收治的病理诊断明确的60例NK-NPC活检标本,采用免疫组织化学技术(Envison二步法)检测LMP-1、NET-1、VEGF的表达,并分析其与临床病理参数和预后的相关性。另取10例鼻咽慢性黏膜炎症组织作对照。结果:(1)NK-NPC中LMP-1、NET-1、VEGF的表达显著高于鼻咽慢性黏膜炎症组织(P<0.05);(2)NK-NPC中, LMP-1表达强度与有无远处转移密切相关(P<0.05),与患者性别、年龄、T分期、N分期无显著相关性(P>0.05);NET-1、VEGF表达强度与淋巴结转移,远处转移密切相关(P<0.05),与患者性别、年龄、T分期均无显著相关性(P>0.05)。(3) NK-NPC中LMP-1、NET-1、VEGF的表达呈显著相关性(P<0.05)。(6)单因素Kaplan-Meier生存曲线分析显示,LMP-1、VEGF表达与NK-NPC的生存率之间的相关性有显著统计学意义(P<0.05)。结论:LMP-1、NET-1、VEGF的联合检测有助于预测NPC转移与预后,LMP-1和VEGF可作为评估NK-NPC预后的指标之一。
Objective：To investigate the expression of latent membrane protein-1 （LMP-1） , NET-1, and vascular endothelial growth factor （VEGF） in non-keratin nasopharyngeal carcinoma （NK-NPC） and its clinical significance. Methods： Sixty biopsy specimens from pathologically-confirmed NK-NPC patients, who were treated in the Affilitated Hospital of Nantong University from May 1999 to May 2003, were included in the present study. Using immunohistochemical techniques （Envison two-step） , we examined the expression of LMP-1, NET-1 and VEGF protein in the specimens. The relationship between their expression and clnicopathological parameters and the prognosis was anayzed. Ten specimens of chro- nic nasopharyngitis served as control. Results ： （ 1 ） The positive rates of LMP-1, NET-1 and VEGF in NK-NPC were significantly higher than those in the chronic nasopharyngitis （P 〈 0.05 ）. （2） The LMP-1 expression in NK-NPC specimens were positively correlated with distant metastasis of NK-NPC （ P 〈 0.05 ）, and not correlated with the sex, age, T stages and N stages （P 〉 0.05）. （3） NET-1 and VEGF expression in NK-NPC specimens were positively correlated with lymph node metastasis and distant metastasis （P 〈 0.05）, and not correlated with the sex, age, and T stages. （4） The expression of LMP-1, NET-1, and VEGF was correlated with each other （P 〈 0.05 ）. （5） Kaplan - Meier survival curve analysis showed that the expression of LMP-1, VEGF was significantly correlated with the prognosis of NK-NPC patients （P 〈 0. 05）. Conclusion： Combined examination of LMP-1, NET-1, and VEGF can help to predict NPC metastasis and prognosis. LMP-1 and VEGF might be used as an indicator for prognosis of NK-NPC.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
2008年第6期575-580,共6页
Chinese Journal of Cancer Biotherapy
关键词
非角化性鼻咽癌
潜活膜蛋白
NET-1
血管内皮生长因子
预后
non-keratin nasopharyngeal carcinoma（NK-NPC）
latent membrane protein 1 （ LMP-1 ）
NET-1
vascularendothelial growth factor（VEGF）
prognosis
