摘要
目的探讨脐带问充质干细胞（UC—MSCs）对MRL／lpr狼疮鼠的治疗作用。方法24只18周龄雌性MRL／lpr小鼠，分为3组：UC～MSCs1次治疗组（G1）、UC—MSCs3次治疗组（G2）、对照组（G3）。观察体质量，考马斯亮蓝法检测尿蛋白定量（24h），酶联免疫吸附法（ELISA）检测抗双链DNA（dsDNA）抗体水平，观察肾脏、肺病理改变。结果①尿蛋白定量（24h）25周时G1组（2．3±1．9）mg和G2组（1．8±1．4）mg显著低于对照组（3．8±2．1）mg（P〈0．05），27周时G1组（2．5±1．5）mg和G2组（1．9±1．2）mg也显著低于对照组（5．4±2．4）mg（P〈0．01）。②24周时治疗组体质量显著高于对照组（P〈0．05），29周时血清肌酐G1组（7．2±3．2）μmol／L和G2组（6．2±2．8）μmol／L显著低于对照组（12．5±2．3）μmol／L （P〈0．05）。③移植1周时，抗dsDNA抗体滴度G1组（46±11）×10^2U／ml和G2组（49±43）×10^2U／ml显著低于对照组（99±42）×10^2U／ml（P〈0．05）；29周时G2组（36±15）×10^2U／ml显著低于对照组（68±32）×10^2U／ml。④肾小球新月体形成率G1组（0．12±0．07）和G2组（0．08＋0．02）显著低于对照组（0．20±0．06）（P〈0．05），G2组较G1组显著减低（P〈0．05）。⑤间质性肺炎G1组和G2组较对照组减轻。结论UC—MSCs对MRL／1Dr狼疮鼠有显著疗效，安全且无排斥反应。
Objective To investigate the efficacy of umbilical cord mesenchymal stem cells （UC- MSCs） transplantation in the treatment of the MRL/Ipr mice. Methods Twenty four 18-week-old MRI/lpr female mice were divided into 3 groups ： group 1 （G1） were transplanted with 1×10^6 UC- MSCs through caudal vein, group 2 （G2） were transplanted with 1×10^6 UC- MSCs three times and group 3 （G3） were treated with 0.5 ml normal saline as controls. Enzyme linked immunosorbent assay （ELISA） was used to measure the levels of serum anti-dsDNA antibodies. Twenty-four hours proteinuria and body weight were assessed every two weeks. The histopathology changes of the kidneys and lungs were observed. Results ① At the 25th weeks, the 24 hours proteinuria in group G1 （2.3±1.9） mg and G2 （1.8±1.4） mg was decreased than that in the control group （3.8±2.1） mg （P〈0.05）, and at the 27th weeks, that of groups G1 （2.5±1.5） mg and G2 （1.9±1.2） mg was also significantly decreased than in the control group （5.4±2.4） mg （P〈0.01）; ② From the 24th week, the body weight of groups G1 and G2 increased significantly than that of the control group （P〈 0.05）. At week 29, serum creatinine decreased significantly in both groups G1 （7.2±3.2） μmol/L and G2 （6.2±2.8） μmol/L than in the control group （12.5±2.3） μmol/L （P〈0.05）; ③ One week after transplantation, the levels of anti-dsDNA antibodies in group G1 （46±11）×10^2U/ml and G2（49±43）×10^2U/ml were both significantly decreased than those of the control groups （99±42）×10^2U/ml （P〈0.05） and the difference between group G2 （ 36± 15 ） ×10^2U/ml and the controls （ 68 ±32 ） ×10^2U/ml was statistically significant; ④ The nephron crescent formation in group G1 （0.12±0.07） and G2 （0.08±0.02） was significantly lower that of the control group （0.20±0.06） （P〈0.05） and that of group G2 was significantly less that of froup G1 （P〈0.05） ; The interstitial pneumoniti
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2009年第1期4-7,I0001,共5页
Chinese Journal of Rheumatology
基金
国家自然科学基金（30772014）
教育部高校博士点专项基金（20050315001）
江苏省科教兴卫工程重点人才基金
南京市科技发展项目基金（200702068）
关键词
脐带
间质干细胞移植
红斑狼疮
系统性
小鼠
近交MRL
LPR
新月体形成指数
Umbilical cord
Mesenchymal stem cell transplantation
Lupus erythematosus, systemic
Mice, inbred MRL lpr
Crescent formation index
