摘要
目的探讨系统性红斑狼疮(SLE)患者骨髓间质干细胞(MSCs)生物学功能、细胞因子的表达异常与否。方法采用密度梯度离心和贴壁筛选法对10例SLE患者和11例正常人骨髓MSCs进行分离培养,流式细胞仪鉴定MSCs表面标志物及分析MSCs细胞周期和细胞凋亡,观察MSCs形态学改变,MTT法检测MSCs的生长情况,RT-PCR检测MSCs细胞因子mRNA水平表达。结果SLE骨髓MSCs传代率(30%)低于正常(100%)(P<0.01),SLE患者骨髓MSCs生长较正常活跃,凋亡率(2.36%)低于正常(6.79%),两组MSCs均表达CD29、CD44、CD105,均不表达CD14、CD34、CD45,SLE患者骨髓MSCsIL-7mRNA表达低于正常。结论SLE骨髓MSCs生物学特性及细胞因子表达存在异常。
Objective To explore the biological characters and cytokine expression of bone marrow mesenchymal stem cells （MSCs） from patients with systemic lupus erythematosus （SLE）. Methods MSCs were isolated from bone marrow of both 10 SLE patients and 11 normal humans by density centrifugation and adhesive culture in vitro. The surface markers, cell cycle and cell apoptosis were detected by flow cytometry （FCM）. The morphological change of MSCs was observed in primary and passage cultures. The proliferation of cells was determined by MTT colorimetric assay. The mRNA expression of cytokine secreted from MSCs was analysed with RT-PCR. Results The ex vivo passage rates of MSCs were 30% in SLE patients and 100% in normal controls, respectively （P〈0.01）. MSCs from SLE grew faster than normal controls. The apoptotic percentage of MSCs was 2.36% in SLE and 6.79% in normal controls. Both groups express CD29, CD44 and CD105, but no express CD14, CD34 and CD45. IL-7 mRNA level of MSCs in SLE was less than the normal controls. Conclusion MSCs of SLE patients present the abnormalities in both biological characters and cytokine expression.
出处
《中华风湿病学杂志》
CAS
CSCD
2005年第10期586-589,共4页
Chinese Journal of Rheumatology
基金
江苏省135重点人才培养基金资助项目(RC2002003)
南京市卫生局重点项目(ZKG9905)
