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骨髓来源细胞在脉络膜新生血管生成中的作用 被引量:6

Bone marrow-derived cells and choroidal neovascularization
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摘要 目的探讨骨髓来源细胞(BMCs)在脉络膜新生血管(CNV)生成中的作用。方法将绿色荧光蛋白(GFP)转基因小鼠骨髓细胞移植给野生型C57BL/6J小鼠建立嵌合体,应用532nm倍频激光诱发嵌合体小鼠(处理组)和野生型小鼠(对照组)CNV。利用脉络膜铺片观察由GFP阳性细胞形成的新生血管;免疫荧光染色观察CNV中GFP阳性细胞是否表达血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)。结果激光光凝后第29天,脉络膜铺片显示CNV中有大量绿色的骨髓来源细胞,由其组成的血管面积约占CNV表面积的16.22%;免疫荧光染色结果显示,CNV区域的一些BMCs表达VEGF和bFGF。结论BMCs通过参与新生血管组成及分泌促血管生成因子的方式,可能在CNV发生过程中发挥着重要作用。 Objective To investigate the role of bone marrow-derived cells (BMC) plays in choroidal neovascularization (CNV). Methods Green fluorescent protein (GFP) chimeric mice were built by transplanting BMC from GFP transgenic mice to adult wild type C57BL/6J mice. Retinal laser photocoagulation was used to induce CNV in the chimeric mice (treated group) and adult wild type mice (control group). Four weeks later, choroidal flatmount was prepared to detect GFP positive BMC expression in the CNV lesions, and immunofluoreseence stain was used to determine the expression of vascular endothelia growth factor (VEGF) and basic fibroblast cell growth factor (bFGF). Results Twenty-nine days after pbotocoagulation lots of GFP-positive BMC presented in the CNV area, which accounted approximate 16.22% of the total CNV area. Some BMC in the CNV area expressed VEGF and bFGF. Conclusions BMC may play an important role in CNV by forming new vessles and secreting angiogenic factors.
出处 《中华眼底病杂志》 CAS CSCD 北大核心 2009年第1期30-33,共4页 Chinese Journal of Ocular Fundus Diseases
基金 国家自然科学基金(编号:30371516,30672291) 教育部留学回国人员科研启动基金资助项目(2004) 志谢 本研究得到德国洪堡基金会(Alexander von Humboldt Foundation)仪器设备捐赠基金(V-8151/02085,To YS Wang)资助
关键词 脉络膜新生血管化 骨髓细胞 血管生成诱导剂 动物实验 Choroidal neovaseularization Bone marrow cells Angiogenesis inducing agents Choroidal neovaseularization Bone marrow cells Angiogenesis inducing agents Animal experimentation
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参考文献27

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