摘要
目的：探讨HBV e系统双阳模式产生的一般性原因。方法：常规乙肝标志物免疫学检测筛选出61份e系统双阳模式血清样本,对每一份样本进行HBeAg RIA定量、前S1抗原检测以及HBV DNA荧光定量。结果：61份双阳模式血清的HBeAg RIA定量在1.5×105～2.1×105ng/ml区间内;前S1抗原检测54份阳性,阳性率88.5%;HBV DNA荧光定量全部阳性,平均拷贝数在107以上。结论：e系统双阳模式中的HBeAb为假阳性,双阳模式实为病毒高载量的表现,而不是血清转换。
Objective：To discover the commonly mechanism causing simultaneous positive of HBeAg and HBeAb.Methods：A total of 61 serum samples with simultaneous positive of HBeAg and HBeAb were screened out by HBV ELISA routine.In each serum,concentration of HBeAg was measured by RIA,HBV DNA copies was quantitated by FQ-PCR and Pre-S1 antigcn was detected by sandwich immunoassay.Results：In 61 sera,the concentration of HBeAg was 1.5×105 to 2.1×105 ng/ml.Pre-S1 antigcn was detected in 54 sera and the positive rate was 88.5%.HBV DNA was positive in all 61 sera,the average of copies was above 107.Conclusion：The positive of HBeAb in pattern of simultaneous positive of HBeAg and HBeAb are usually false,and the pattern represents high load of virus,but not serum-transition.
出处
《中国卫生检验杂志》
CAS
2008年第12期2641-2642,共2页
Chinese Journal of Health Laboratory Technology
关键词
乙型肝炎
双阳模式
假阳性
Hepatitis B virus
Simultaneous positive of HBeAg and HBeAb
False positive
