摘要
目的：研究维生素Ｅ（ＶｉｔＥ）的镇痛作用及机制。方法：在小鼠热板和扭体反应模型上，观察用药后舔足潜伏期和扭体数改变，丙二醛（ＭＤＡ）用巴比妥酸法测定，一氧化氮含量用Ｇｒｅｅｎ法测定。结果：１００，２００ｍｇ·ｋｇ－１ＶｉｔＥｉｍ可显著延长小鼠舔足潜伏期和减少小鼠扭体数，并且１００ｍｇ·ｋｇ－１ＶｉｔＥ的镇痛作用可被ＥＧＴＡ加强，ＣａＣｌ２所拮抗，但维拉帕米可部分逆转ＣａＣｌ２对ＶｉｔＥ镇痛的拮抗；ＶｉｔＥ产生镇痛时，也可显著抑制小鼠脑组织中ＭＤＡ和ＮＯ含量增高，并可被ＥＧＴＡ增强，ＣａＣｌ２所减弱。结论：上述结果表明ＶｉｔＥ有显著的镇痛作用，其作用与减少脑内ＮＯ和ＭＤＡ生成有关，并被Ｃａ２＋所影响。
OBJECTIVE: To study the analgesic effect of vitamine E and its mechanism. METHODS: On the hotplate and writhing test in mice, the changes of the latencies of licking paws and the writhing numbers were observed after drug administration. The content of malondialdehyde (MDA) was measured with thiobarbituric acid method. Green method was used for the determination of nitric oxide (NO) content. RESULTS: 100, 200 mg·kg-1 vitamin E could significantly prolong the latencies of licking paws and reduce the writhing numbers. The analgesic effect of vitamin E could be antagonized by CaCl2 and enhanced by EGTA. Verapamil partially overturned the antagonism of CaCl2 on the analgesic effect of vitamin E. The increase of NO and MDA contents in the cerebrum was inhibited by vitamin E when analgesic effect of vitamin appeared. This inhibitory effect could also be weakened by CaCl2 and enhanced by EGTA. CONCLUSION: Vitamin E has significant analgesic effect and its effect related to the reduction of MDA and NO in brain and was influenced by calcium ion.
出处
《中国药学杂志》
CAS
CSCD
北大核心
1998年第5期285-288,共4页
Chinese Pharmaceutical Journal
关键词
维生素E
镇痛
药理学
vitamin E, analgesic effect, calcium ion, nitric oxide, malondialdehyde
