摘要
为了探讨Ｆａｓ／ＡＰＯ１在泌尿系恶性肿瘤发生发展中的作用，采用免疫细胞化学方法对６种泌尿系肿瘤细胞，膀胱癌（Ｔ２４，ＥＪ，ＢＩＵ８７），肾癌（ＧＲＣ１，ＲＣＣ９４９），前列腺癌（ＰＣ３Ｍ）和一种原代培养正常肾间质成纤维细胞Ｆａｓ／ＡＰＯ１分子的表达进行了检测。结果显示原代培养正常肾间质成纤维细胞和Ｔ２４细胞系Ｆａｓ／ＡＰＯ１分子表达较强，ＰＣ３Ｍ属中等强度表达，ＲＣＣ９４９与ＢＩＵ８７属微弱表达，而ＥＪ及ＧＲＣ１则不表达。Ｆａｓ／ＡＰＯ１在泌尿系恶性肿瘤培养细胞中的表达强度偏低或完全不表达，可能参与了泌尿系恶性肿瘤的发生和发展。
To explore the role of Fas/APO1 in the occurrence and progression of human urinary malignant tumors, expression of Fas/APO1 was tested with immunocytochemical technique in 6 kinds of urinary tumor cell lines consisting of bladder cancer (T24, EJ, BIU87), renal cell carcinoma (GRC1,RCC949),prostatic cancer (PC3M) and a primary in vitro cultured normal renal fibroblast. The results showed that expression of Fas/APO1 was strong in T24 cell line and in primary in vitro cultured renal fibroblast, medium in PC3M cell line, weak in RCC949 and BIU87 cell lines and negative in GRC1 and EJ cell lines. Expression of Fas/APO1 in malignant urinary tumor cell lines was relatively low or negative which may be related to the occurrence and progression of urinary malignant tumors.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
1998年第5期303-305,共3页
Chinese Journal of Urology
基金
国家自然科学基金
