摘要
目的 :探讨前列回春对实验性大鼠前列腺组织Fas表达及细胞凋亡的影响。方法 :采用大鼠去势后注射丙酸睾酮引起前列腺增生法造模 ,灌胃给药 3 0d后 ,处死大鼠取前列腺组织并称湿重 ,用免疫组化方法检测前列腺组织中Fas阳性细胞的表达率 ,用流式细胞术检测细胞凋亡率和观察细胞凋亡峰。结果 :前列回春各组前列腺组织湿重与模型组比较差异非常显著 (P <0 .0 1) ;前列回春各组和雌二醇组前列腺组织中Fas表达率明显高于模型组 (P <0 .0 5,P <0 .0 1) ;与正常组和模型组比较 ,前列回春和雌二醇组的细胞凋亡率明显提高 ,差异有显著性(P <0 .0 1)。结论 :前列回春可增加实验性大鼠前列腺组织中Fas的表达 ,促进大鼠前列腺组织细胞凋亡 ,降低大鼠前列腺组织的重量 ,而且呈明显量效关系 。
Objective: To investigate the effect of Qi anlie Huichun capsular on Fas expression and cell apoptosis of prostate gland tissue in the model rat. Met hod: Hypertrophy of prostate model rat was established by injecting t estosterone to gelding male rats. After being treated with Qianlie Huichun ig 30 days, the rats were killed and prostate glands were resected for examination. The Fas expressio n was examined by immunobistochemical SABC. The cell apoptosis and the peak of cell apoptosis in the prostate gland of the rats were examined by flow cytometry. Compared with model group,the weight of prostage gland tiss ue in groups treated with Qianlie Huichun capsular was light ( P<0.01 ). Result: Compared with model group, the Fas expression i n all treatment group s increase( P<0.05,P<0.01). Compared with normal and model groups, cell apoptosis i n all tr eatment groups increase at different level( P<0.01). Conclusion: The Qianlie Huchun capsular increases the Fas expression and cell apoptosis of model rats, a nd shows a definite treatment effect on the h ypertrophy of prostate by promoting the apoptosis of prostate cell.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2003年第9期866-869,共4页
China Journal of Chinese Materia Medica
