摘要
目的研究[Gly^14]-humanin（HNG）对脑出血（ICH）模型大鼠的神经保护作用。方法将健康雄性SD大鼠30只采用随机数字表法分为HNG治疗组（10只）、模型组（10只）、假手术组（10只），立体定向注射自体血制作ICH模型，假手术组仅插入针，HNG治疗组大鼠在造模后侧脑室注射HNG-72h后观察血肿周围胶质细胞和凋亡细胞的变化。结果术后72h，HNG治疗组星形胶质细胞和小胶质细胞形态与模型组比较胞体小，突起短、细；HNG治疗组血肿周围胶质纤维酸性蛋白GFAP、OX42阳性细胞数以及凋亡细胞数量f105．3±10．19，173．2±21．72，26．1±5．97）较模型组（141．8＋9．77，212．2±15.38．48．6±10．94）明显减少，但仍高于假手术组，差异有统计学意义（P均〈0．05）。结论HNG在大鼠ICH后血肿周围脑组织中具有抗炎症性神经保护作用。
Objective To investigate the neuroprotective effect of [Gly^14]-humanin （HNG） in rats with intracerebral hemorrhage （[CH）. Methods Thirty healthy male SD rats were randomly divided into 3 groups, namely the sham operated group （10 rats） with needle insertion only, 1CH model group （10 rats） with injection of autologous whole blood into the right caudate nucleus, and HNG treatment group （10 rats） with HNG injection into the lateral cerebral ventricle after simulated ICH as in the model group. The changes in the glial cells and cell apoptosis around the hematoma were detected 72 h after the operation. Results The astrocytes and microglial cells in rats receiving HNG injection into the cerebral ventricles showed smaller cell size and shorter and thinner cell processes than those in ICH model group. The numbers of cells positive for glial fibrillary acidic protein （GFAP） and OX42 and the apoptotic cells （as found by TUNEL assay） around the hematoma were significantly reduced in comparison with those in the ICH model group （P〈0.05）, but still remained significantly higher than those in the sham-operated group （P〈0.05）. Conclusion HNG can ameliorate the inflammatory response occurring in and around the hematoma and provide some neuroprotection in rats with ICH.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2009年第1期19-22,共4页
Chinese Journal of Neuromedicine
关键词
HNG
脑出血
小胶质细胞
细胞凋亡
[Gly^14]-humanin
Intracerebral hemorrhage
Microglia
Apoptosis
