摘要
目的探讨5-FU(5-氟尿嘧啶)联合热疗诱导SW480细胞(人结直肠癌细胞株)的增殖抑制率、细胞周期各时相的影响及亚细胞结构改变。方法应用MTT比色法检测SW480细胞的增殖抑制率,流式细胞仪检测细胞周期各时相的变化,电子显微镜观察亚细胞结构变化。结果联合热疗组与5-FU组、热疗组相比差异非常显著,P<0.01。且SW480细胞周期进程发生明显改变,G1期细胞增多、S期细胞减少、G2/M细胞相对增多,细胞凋亡率升高。电子显微镜结果显示染色质趋边凝集、线粒体膜、溶酶体膜、内质网膜系统明显改变,可见凋亡小体。结论5-FU联合热疗可显著提高SW480细胞增殖抑制率,抑制SW480细胞G1期向S期转化进程,诱导细胞凋亡及亚细胞结构改变。
Objective To investigate proliferation suppressing rate , the effect of cell cycle phases and sub-cytoarchitecture changes induced by 5-Fu combined with Hyperthemaia in colon cancer SW480 cells. Methods MTr assay and flow cytometry were applied to detect the proliferation suppressing rate of SW480 cell and the change and apoptosis rate of cell cycle phases. Using electron microscope to observe the change of sub-cytoarchitecture induced and differentiated by 5-Fu combined with Hyperthermia. Results Significant difference can be observed in Group 5-Fu combined with Hyperthermia,group 5-Fu and group Hyperthemaia P 〈 0.01. Moreover,significant changes can be observed during SW480 cell cycle process that phase G1 cell number rising,phase S reduction, G2/Mcell relative increase and the rate of apoptesis increase.Electron microscope showed that chrornatin ehemokine-lectin,the mitochondrial membrane, lysosomal membrane, and the reticulum system change ignificanfly. In addition, this shows that apoptotie bodies. Conclusion 5-Fu combined with Hyperthermia can signi/icantly improve SW480 cell proliferation suppressing rate, hold back cell cottrse from G1 to S,and induce cells apoptesis and the change of sub-cytoarchitecture.
出处
《中国实验诊断学》
北大核心
2009年第1期30-32,共3页
Chinese Journal of Laboratory Diagnosis
