摘要
目的构建人IKKα、IKKβ基因RNA干扰慢病毒质粒,并观察其对肝癌细胞生长的影响。方法将目的片段亚克隆入慢病毒载体中,包装成病毒后感染人肝癌细胞,检测细胞IKKα、IKKβ的表达,并将感染细胞接种于裸鼠皮下,观察肿瘤生长情况。结果目的片段被成功克隆入载体质粒。pLenti-GFP-IKKα-SiRNA慢病毒液感染的细胞中IKKα蛋白表达明显降低,pLenti-GFP-IKKβ-SiRNA慢病毒液感染的细胞中IKKβ蛋白表达明显降低。实验组裸鼠皮下肿瘤出现晚且生长缓慢。结论成功构建了针对人IKKα、IKKβ基因RNA干扰慢病毒质粒,包装成病毒后感染人肝癌细胞后能抑制其生长。
Objective To construct lentiviral vector mediated SiRNA targeting IKKα and IKKβ gene and explore its effects on the growth of hepatocellular carcinoma （HCC） cells. Methods Two recombinant vectors were generated by subcloning a target DNA into lentiviral vector. After being infected with the virus, the expression of IKKa and IKKβ in HCC cells was examined. The transfected cells were also injected into mice subcutaneously, and the growth of tumor was observed. Results The expression of IKKα and IKKβ protein in transfected cells decreased, and tumor growth inhibition was observed obviously in mice. Conclusion Lentiviral vector transduced small interference RNA targeting IKKα and IKKβ gene can suppress the growth of HCC cells.
出处
《江苏医药》
CAS
CSCD
北大核心
2009年第1期75-77,共3页
Jiangsu Medical Journal
基金
国家自然科学基金(30672367)
卫生部科研基金(WKJ-2006-2-21)
江苏省医学重点人才(RC2007057)
江苏省六大人才高峰项目(06-B-032)
