摘要
以锯缘青蟹Scylla serrata为研究对象,运用亲和层析、聚丙烯酰胺凝胶电泳(PAGE)、十二烷基璜酸钠(SDS)-PAGE、Western-blot以及酚氧化酶活性测定等方法研究了锯缘青蟹血蓝蛋白的酚氧化酶活性以及几种金属离子对其酚氧化酶活性的影响.研究结果表明,锯缘青蟹血蓝蛋白在没有任何诱导物作用的情况下表现出酚氧化酶活性,胰蛋白酶对此活性有轻微抑制作用;与对照组相比,Ca2+和Cu2+能使血蓝蛋白酚氧化酶活性显著增强,增强率分别为281%和273%;EDTA可显著抑制Cu2+对血蓝蛋白酚氧化酶活性的激活作用,抑制率达78.5%.由此可知,锯缘青蟹血蓝蛋白确有酚氧化酶活性,且受Ca2+、Cu2+等金属离子作用的影响.
To investigate the phenoloxidase activity of hemocyanin from crab, Scylla serrata, and the effects of some metallic ions on it, the methods of affinity chromatography, PAGE, SDS-PAGE, Western- blot and phenoloxidase activity measurement were used. The results indicated that hemocyanin showed phenoloxidase activity without any inductor and trypsin could inhibit the activity slightly. Compared with control group, Ca^2＋ and Cu^2＋ could enhance its phenoloxidase activity at an enhancement rate of 281% and 273%, respectively; EDTA could inhibit the activation by Cu^2＋ remarkably at an inhibitory rate of 78.5%. It is concluded that hemocyanin of Scylla serrata actually had phenoloxidase activity which was influenced by Ca^2＋ and Cu^2＋.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2009年第1期46-50,共5页
Journal of South China Agricultural University
基金
广东省自然科学基金(06027212)
汕头市科技计划项目(2006-148)
