摘要
根据GenBank上发表的牛IL-2(M13204)基因序列,应用Primer Premier5引物设计软件自行设计一对引物,以ConA刺激培养24h的奶牛外周血淋巴细胞为材料,从总RNA中扩增出奶牛IL-2基因,然后测序。结果表明克隆的奶牛IL-2基因大小477bp,编码158个氨基酸,与GenBank上发表的牛IL-2基因序列比较,其核苷酸的同源性99.4%,与马、猪、猫、狗、山羊、绵羊的核苷酸的同源性分别为31.8%,32.0%,31.6%,30.3%, 30.8%,31.0%。
According to cattle IL -2 （M13204） gene sequence published by GenBank, a pair of primer was designed by primer premier 5 software, then A 477bp DNA fragment was amplified by RT -PCR technology using total RNA template extracted from Chinese Holstein Cattle blood lymphocyte that stimulate 24 hour by 1 milligram per milliliter concentration ConA. Agarose gel electrophoresis showed that the DNA fragment is about 477bp, the nucleotide homology between cloning cow IL - 2 and cattle IL - 2 reported by GenBank was 99.4%. The IL - 2 gene homology between cow and horse, pig, cat, dog, goats, sheep was respectively 31.8 %, 32.0,31.6,30. 3%, 30.8%, 31.0%.
出处
《山东农业大学学报（自然科学版）》
CSCD
北大核心
2009年第1期49-53,共5页
Journal of Shandong Agricultural University：Natural Science Edition
基金
江河学院生物仪字与分子生物学重点学科建设项目(071010)
江河学院博硕科研启动项目(XS205026)
