摘要
建立了一种以金纳米微粒为探针共振光散射(RLS)法测定维生素B_4的新方法。在弱酸性介质中(pH 4.2),金纳米微粒在635 nm有一最大共振散射峰。加入微量维生素B_4后,金纳米微粒与维生素B_4通过静电引力结合,形成了粒径较大的聚集体,导致RLS强度显著增强。研究了体系的共振光散射光谱特征和反应适宜条件,探讨了共振光散射增强的机理。结果表明,维生素B_4质量浓度在0.1～5.0μg/mL时与散射强度(ΔI)呈线性关系,检出限(3σ)为12.0 ng/mL,相对标准偏差(RSD)为2.2%。该方法已用于片剂中维生素B_4的测定。
A sensitive method for detecting Vitamin B4-aqueous adenine was proposed by resonance light scattering （RLS） spectroscopy with gold nanoparticles as probe. In a weak acidic medium （pH 4.2）, the resonance light scattering spectrum of gold nanoparticles had a maximum peak at 635 nm and the RLS intensity was enhanced by trace amount of Vitamin B4 due to forming aggregate with bigger diameters between gold nanoparticles and Vitamin B4. The RLS spectral characteristics of gold nanoparticles-Vitamin B4 system and the optimum conditions of the reaction were investigated. The mechanism for the enhancement of RLS was also discussed. The results showed that the enhancement of the RKS signal （△I） was proportional to the concentration of Vitamin B4 in the range of 0.1 - 5.0μg/mL. The corresponding detection limit （3σ） was 12 ng/mL, and the RSD was 2.2%. The method was applied to the determination of Vitamin B4 in tablets with satisfactory results.
出处
《分析试验室》
CAS
CSCD
北大核心
2009年第1期48-52,共5页
Chinese Journal of Analysis Laboratory
基金
陕西省自然科学基金(2003B09)项目资助
