摘要
目的研究食管癌中是否存在螺杆菌感染及其与炎性信号通路的关系，探讨螺杆菌感染以及所产生的慢性炎症在人食管癌发生发展中的作用。方法随机收集食管癌标本37例和正常食管标本10例，采用聚合酶链反应（PCR）扩增螺杆菌16S rDNA，并进行基因测序及与幽门螺杆菌（Hp）同源性比较。阳性者再扩增Hp特异性毒力基因（cagA，babA2，vacA）。随后将Hp标准株NCTC11639和NCTC11637分别与食管鳞癌细胞株EC109共培养不同的时间，蛋白印迹（Westem blot）检测炎性信号通路中P—IκB—α蛋白表达的变化。结果食管癌组织中螺杆菌16S rRNA基因的检出率为31．6％（12／37），正常人食管组织均元阳性表达。对阳性标本16S rRNA基因测序及同源性比较，食管癌组织中螺杆菌序列与Hp的16S rDNA序列同源性达99％～100％。12例阳性标本中分别有cagA阳性4例，babA2阳性8例，vacA阳性3例，阳性率分别为33％，67％，25％。EC109与Hp标准株共培养不同时间后，P—IκB-α显著升高。结论食管癌组织中存在螺杆菌感染，该螺杆菌可能为Hp或者其他亚种。螺杆菌感染导致食管粘膜慢性炎症在食管癌的发生发展中可能发挥重要的作用，其中P—IκB-α炎性信号通路的持续作用可能为分子机制之一。
Objective To explore the effect of helicobacter infection and its rink to inflammatory signalling pathway in esophageal carcinoma and the relationship between chronic inflammation and esophageal carcinoma. Methods Thirty - seven samples of esophageal carcinoma and 10 samples of normal esophageal tissues were randomly collected. Helicobacter species were examined by polymerase chain reaction （PCR） with helicobacter - specific 16SrDNA primers. The target products amplified were sequenced and compared with helicobacter. A research for important virulence genes （cagA, babA2, vacA） specific for helicobacter pylori（Hp） were also carried out by PCR. The standard strain of Hp was co - cultured with EC109 for different period of time and P - IκB - α was detected with Western blot. Results Helicobacter species were found in esophageal tissue in 31.6% （ 12/37 ） of patients with squamous carcinoma whereas none of the controls harboured the 16SrDNA. Nine of helicobacter - specific PCR amplicons were sequenced and the homology was 99% - 100% to 16SrDNA of Hp. Virulence genes ,4 cases of cagA ,8 cases of babA2 and 3 cases of vacA were found in the helicobacter 16SrDNA - positive esophageal squamous carcinoma. Expression of P - IκB - α in the EC109 markedly increased after co - cultured with Hp. Conclusion Helicobacteraceae are found in human esophageal carcinoma tissues which shows the infection of helicobacter species likely Hp. The infection of the bacteria might play a very important role in human esophageal carcinoma generation and development,in which P - IκB - α - associated inflammatory signalling pathway might be one of molecular mechanisms of persisting inflammation.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2009年第1期90-92,共3页
Chinese Journal of Public Health
