摘要
C2H2锌指是真核细胞中最常见的DNA结合模体.对通过mRNA差异显示技术和克隆测序,在褐牙鲆中获得的一条白化相关锌指蛋白基因zfp123,进行了电子表达谱与实验验证表达谱的比较分析及该蛋白序列的一、二级和三级结构分析.电子表达谱分析分别对与ZFP123有较高同源性的斑马鱼、家鼠和猪的zfand5a(zfp123)基因各个组织表达情况进行了比较分析;一、二级结构分析发现其具有多个保守的基序和结构域;同源建模显示其具有一个"C-X(2-4)-C-X11-C-X2-C"结构的锌指样三级结构.ZFP可以介导靶基因的转录调控,抑制或激活特定基因的表达;对DNA进行修饰,如人造限制性内切酶、重组酶、抗病毒感染等.锌指蛋白应用前景广阔,研究价值显著,是未来基因治疗的革命性的工具.
C2H2 zinc finger is one of the most common eukaryotic DNA binding motifs. An albinism-related gene of zfp123 of Paralichthys olicaceus was acquired by mRNA differential display, cloned and sequenced. The comparative analysis of electronic expression profile and experimental verification expression profile, and the analyses of the primary, secondary and tertiary structures of the protein were performed. The comparative analysis of electronic expression profile of zfand5a gene from zebrafish, house mouse and pig that has high sequence homologies with zfp123 gene from P. olicaceus were carried out. Several conserved domains and motifs were discovered by the primary and secondary structure analyses. Zinc finger- like tertiary structure ＂C-X（2-4）-C-X11-C-X2-C＂ was shown by homology modeling. Zinc finger protein is frequently used for transcription regulation, which activates or inhibits specific gene expression, DNA modification （such as artificial restricted endonuclease and recombinase） and virus infection repression. Accordingly, zinc finger protein is broadly used and highly valued for scientific research, bio-medicine production and clinic therapy, especially for gene therapy, which may become the evolutional tool in the future. Fig 2, Tab 1, Ref21
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2008年第6期779-782,共4页
Chinese Journal of Applied and Environmental Biology
基金
辽宁省科技基金资助(No.2050819)项目~~
关键词
褐牙鲆
白化相关基凶
组织表达谱
蛋白结构
Paralichthys olicaceus
albinism-related gene
tissue expression profile
protein structure
