摘要
研究了复合酶(纤维素酶和果胶酶)提取白术多糖的最佳工艺条件。分别比较了酶解温度、酶解pH、酶解时间以及酶量对多糖提取率的影响。并采用正交设计优化提取条件,确定最佳提取工艺:酶解温度为50℃,酶解pH为5.0,酶解时间为50 min,复合酶量为0.7%。在最佳提取工艺下,多糖的得率为43.00%。
Composite enzymatic（cellulase and pectase） extraction of atractylodes macrocephala polysaccharides was studied in the paper. The main factors that affect extraction yield in the final product were analyzed, such as enzyma reaction temperature, pH, time and enzyma concentration. By means of orthogonal design, optimum parameters were confirmed as enzyma reaction temperature 50℃ , enzyma reaction pH 5.0, enzyma reaction time 50 min and enzyma concentration 0.7 %. Under these conditions, the polysaccharides extraction rate was 43.00 %.
出处
《广东化工》
CAS
2008年第12期91-94,共4页
Guangdong Chemical Industry
基金
广东省科技计划项目(2006B20301052)
关键词
白术多糖
纤维素酶
果胶酶
提取
atractylodesmacrocephal apolysaccharides
cellulase
pectase
extraction
