摘要
目的探讨不同放置时间对烟蒂上DNA含量及STR分型的影响。方法以10名志愿者的40枚"红双喜"烟蒂为检材(每人4枚),用chelex-100法分别在第1、4、7和10周时提取烟蒂样本外层烟纸和海绵上的DNA,用Quantifiler人类DNA定量试剂盒进行DNA定量,并同时用AmpFL STR Profiler和IdentifilerTM试剂盒(AB公司)进行PCR扩增及STR分型。结果放置第1、4、7和10周的烟蒂烟纸DNA质量浓度范围分别为0.104～2.52、0.110～2.41、0.0960～2.32和0.0850～2.28ng/μL,16个基因座检出率分别为100%、90%、75%和62.5%。海绵DNA质量浓度范围分别为0.0180～2.40、0.0171～2.25、0.0165～2.15和0.0160～2.15ng/μL,16个基因座检出率分别为97.5%、82.5%、50%和12.5%。结论放置时间对烟纸和海绵上的DNA含量影响不大,对STR分型影响显著,STR分型受海绵的影响比烟纸明显。放置时间越久,检出率越低。
Objective To explore the effect on DNA quantification and STR typing from cigarette butts collected at different time points. Methods Forty ＂Hongshuangxi＂ brand cigarette butts smoked by ten different individuals（4 cigarettes per individual） were collected. DNA was extracted from the outer layer and the sponge of the cigarette butts using chelex-100 extraction kit, as well as STR typing and DNA quanti- tation were simultaneously performed in 1, 4, 7 and 10 weeks, respectively. Results The DNA quantities extracted from the outer layer at the 1st, 4th, 7th and 10th week were 0.104-2.52, 0.110-2.41, 0.0960- 2.32 and 0.085 0-2.28 ng/μL, while the detection rates for 16 loci by STR typing were 100%, 90%, 75% and 62.5%, respectively. The DNA quantities extracted from the sponge were 0.0180-2.40, 0.017 1-2.25, 0.0165-2.15 and 0.0160-2.15 ng/μL, while the detection rates for 16 loci by STR typing were 97.5%, 82.5%, 50% and 12.5%, respectively. Conclusion There is little difference in DNA quantity between the outer layer and the sponge of butts during 10 weeks, but there is an obvious effect on STR typing with prolonged extracting time. There is a much more effect on the sponge than on the outer layer, and the longer the standing time is, the lower the detection rate is.
出处
《法医学杂志》
CAS
CSCD
2008年第6期423-424,428,共3页
Journal of Forensic Medicine
基金
广东省重点科技攻关项目(2005B33701008)
公安部应用创新项目(2005yycxGDST136)
