摘要
背景与目的:研究细胞分裂周期蛋白6(CDC6)、三叶因子2(TFF2)在宫颈癌组织中的表达及其与人乳头状瘤病毒(HPV)16/18感染的相关性,以探讨宫颈癌的发生机制。材料与方法:选择50例宫颈癌石蜡标本、宫颈上皮内瘤变(CIN)Ⅰ20例、CINⅡ-Ⅲ20例、正常宫颈组织20例,采用免疫组织化学方法检测CDC6、TFF2的表达,同时采用PCR技术检测HPV16/18DNA,并对HPV16/18感染情况与CDC6和TFF2表达的相关性进行分析。结果:在宫颈癌组织中CDC6的阳性率高于CIN和正常宫颈组织,差异具有统计学意义(P<0.05),且CDC6阳性表达率与病理分级、淋巴结转移相关,差异具有统计学意义(P均<0.05)。在宫颈癌组织中TFF2的阳性率低于CIN和正常宫颈组织,差异具有统计学意义(P<0.05),TFF2阳性表达率与年龄分组相关,差异具有统计学意义(P<0.05),其阳性表达率均与临床分期、病理分级、淋巴转移无相关关系(P均>0.05);以正常宫颈组织、CIN和宫颈癌为序HPV16/18DNA的阳性检出率逐渐升高,各组间的差异具有统计学意义(P<0.05),但与年龄、临床分期、病理分级、淋巴转移无相关关系(P均>0.05)。在宫颈癌组织中,CDC6与HPV16/18感染呈正相关(rs=0.386,P<0.05),TFF2与CDC6、HPV16/18感染呈负相关(rs=-0.356,P<0.05;rs=-0.500,P<0.05)。结论:宫颈上皮内瘤样病变及宫颈癌组织中CDC6、TFF2表达的改变可能与HPV16/18感染有关。CDC6的异常表达与宫颈癌的恶变程度有关。
BACKGROUND AND AIM： To investigate the relationship between expression of CDC6 and TFF2 and HPV16/ 18 infection in uterine cervical cancer. MATERIALS AND METHODS： Formalin-fixed, paraffin-embedded samples including 50 cases of squamous cell carcinomas, 20 cervical intraepithelial neoplasia （CIN） Ⅱ -Ⅲ and 20 CIN Ⅰ and 20 samples of normal cervical tissues were studied. Using Polymer DetectionSystem For Immuno-Histological Staining （PV-9000） technique, we examined the expression of CDC6 and TFF2 in these samples. The infections of HPV type 16, 18 DNA were determined by PCR. RESULTS： There were significant differences for CDC6 among carcinomas, CIN and normal cervices（P〈0.05） . Expression of CDC6 was correlated with tumor grades and lymph node metastasis （all P 〈 0.05） . The positive expression of TFF2 in carcinomas was significantly lower than in normal cervical tissue and CIN （P 〈 0.05）. The positive rate of TFF2 was associated with age group （P 〈 0.05）but not with tumor grades, clinical stages and lymph node metastasis（all P〉 0.05） .The positive rate of HPV16/ 18 was significantly different between cervical carcinomas, CIN and normal tissues （P 〈 0.05） but not between tumor grades, clinical stages, age group and lymph node metastasis （all P〉 0.05） The expression of CDC6 showed positive correlation with that of HPV16/18 （rs=0.386, P 〈0.05）. HPV16/18 and CDC6 both showed negative correlations with TFF2 （rs= -0.500, P〈 0.05; rs= -0.356, P〈0.05, respectively） . CONCLUSION： In tissues of CIN and cervical carcinomas, changes in CDC6 and TFF2 expression may be associated with HPV16/ 18 infection. Expressions of CDC6 was associated with the malignant level of uterine cervical cancer.
出处
《癌变．畸变．突变》
CAS
CSCD
2008年第6期459-462,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
