摘要
目的了解临床分离的铜绿假单胞菌对碘伏、戊二醛、氯己定、氯氧三嗪(三氯异氰尿酸)和苯扎溴铵5种消毒剂的抗性。方法采用PhoenixTM-100系统鉴定铜绿假单胞菌和药敏试验,用液体稀释法检测碘伏、戊二醛、氯己定、氯氧三嗪和苯扎溴铵对铜绿假单胞菌的最低抑菌浓度(MIC)。结果190株铜绿假单胞菌对氨苄西林/舒巴坦、氯霉素、四环素、复方新诺明耐药率最高均>98.0%,几乎已失去对该菌的杀菌能力;而对亚胺培南、美罗培南耐药率较低,仅为15.3%和6.8%;5种消毒剂中,铜绿假单胞菌对戊二醛和氯氧三嗪的抗性最强,MIC50分别为32μg/ml和64μg/ml;而对氯己定、苯扎溴铵的抗性最弱,MIC50分别为1μg/ml和2.4μg/ml;氯己定在1 MIC作用10 min,仍能产生快速而强烈的杀菌作用。结论铜绿假单胞菌对碘伏、戊二醛、氯己定、氯氧三嗪和苯扎溴铵5种消毒剂的抗性差异有统计学意义,应合理使用消毒剂,以降低耐药菌扩散及医院感染发生。
OBJECTIVE To explore the resistance of Pseudornonas aeruginosa isolated in clinic against five antiseptics involving in povidone iodine（Iodophor）, glutaraldehyde, chlorhexidine, symclosene（trichloroisocyanurate） and benzalkonium bromide. METHODS The susceptibility test of P. aeruginosa was determined by Phoenix^TM-100 system. Minimun inhibitory concentration（MIC） of povidone iodine, glutaraldehyde, chlorhexidine, symclosene and benzalkonium bromide was detected by liquid dilution method. RESULTS The resistant rates of ampicillin/ sulbactam, chloramphenicol, tetracycline and trimethoprim-sulfamethoxazole in 190 isolates of P. aeruginosa were all more than 98.0%. However, P. aeruginosa was to imipenem and meropenem were 15.3% and 6.8%. It was found that P. aeruginosa possessed the most resistant to glutaraldehyde and symclosene with its MIC50 being 32 μg/ml and 64 μg/ml. But the MIC50 of chlorhexidine and benzalkonium bromide were only 1 μg/ml and 2.4 μg/ ml. Meanwhile, time-kill assays indicated that chlorhexidine could still produce rapid and powerful bactericidal effects at a concentration of 1MIC after 10 min treatment. CONCLUSIONS There are distinct differences in P. aeruginosa against povidone iodine, glutaraldehyde, chlorhexidine, symclosene and benzalkonium bromide. It is very important that antiseptics should be used rationally. Measurements should be taken to decrease dissemination of resistant bacteria and prevent nosocomial infection.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2008年第12期1717-1719,共3页
Chinese Journal of Nosocomiology
关键词
铜绿假单胞菌
消毒剂
最低抑菌浓度
Pseudomonas aeruginosa
Antiseptics
Minimun inhibitory concentration
