摘要
目的探讨肝移植术后患者血清中IL-2、IL-6、IL-10、IL-15、VEGF、MCP-1的变化在预测肝移植术后并发症中的作用。方法以2004年6月-2007年1月在本院行肝移植术的79例病人为研究对象,采用ELISA方法检测肝移植患者术前及术后血清中IL-2、IL-6、IL-10、IL-15、VEGF、MCP-1的浓度,观察其变化规律。结果IL-2 GVHD组术前、术后均为0,GVHD组术前、术后均为0,与其它各组比较有统计学意义(P<0.05),血管并发症组术前显著高于其它组(P<0.05),术后各组均升高,重度感染死亡组升高明显,3/5患者>400 pg/ml;IL-6血管并发症组、重度感染死亡组术前较高,而GVHD组术前结果较低,重度感染死亡组术后升高明显(P<0.05),5例患者均>300 pg/ml;其余各组术后均降低;IL-10除排异组、GVHD组降低外,其余各组均升高,其中血管并发症组、重度感染死亡组升高非常明显;IL-15排异组、血管并发症组、重度感染死亡组术后升高(P<0.05);MCP-1术前,对照组明显高于其它组(P<0.05),术后对照组下降,重度感染死亡组升高明显,>300 pg/ml;VEGF二次各组术前、术后数据的测定值几乎均为0。结论IL-2对GVHD的发生有鉴别诊断意义;细胞因子的明显升高,可能预示有重度感染,并濒临死亡;血中细胞因子的检测在预测肝移植术后并发症中有一定的临床意义。
Objective To evaluate the role of IL-2,IL-6,IL-10,IL-15,VEGF,MCP-1 in serum for predicting complications after orthotropic liver transplantation.Methods Seventy-nine case Of liver transplantation from June 2004 to January 2007 in our hospital were observed. The measurements of in serum by ELISA meathed were taken on the last day before operation and the tenth day after operation and the change rules were studied.Results The serum IL-2 levels in GVHD group after operation always kept on 0 levels similar to preoperation, the other groups were significantly higher than before; IL-2 of biliary complication In file vascular complications group before operation were higher than the other groups,the group died of serious infection IL-2 were significantly rising,the level of 3/5 patients IL-2 was higher than 400 pg/ml; before operation, IL-6 of biliary complication In the vascular complications group and the group died of serious infection were higer, but which of group GVHD was lower,after operation,the other groups were decreased,IL-6 were signifieandy rising in the the group died of serious infection; IL-10 in the rejection group and GVHD group were decreased, but which in the vascular complications group and the group died of serious infection were significantly higher than that before transplantation; In the group died of serious infection, rejection group and vascular complications group, IL- 15 were increased compared with preoperation;It observed that MCP-1 in the non-complications group were higer precperafion and were lower after OLT,that which in the group died of serious infection were higher than 300 pg/ml; VEGF in every group were majorly detected 0 pg/ml. Conclusion The serum concentration of IL-2 is kept on 0 pg/ml when GVHD occurred after OLT;whieh most of cytokines in serum were significantly increased is more valuable to the group died of serious infection and indicate that the patient might be on the brink of death; The roles of cytokines in serum have been elucidated in complication after OLT.
出处
《中国实验诊断学》
2008年第12期1513-1516,共4页
Chinese Journal of Laboratory Diagnosis
基金
武警总医院WZ200519课题资助
关键词
肝移植术
细胞因子
并发症
liver transplantation
cytokines
complication
