摘要
目的探讨甲状腺癌前哨淋巴结活检(SLNB)的可行性及临床意义,探索逆转录聚合酶链反应(RT-PCR)技术对诊断前哨淋巴结微转移灶的价值。方法对21例甲状腺癌患者应用亚甲蓝染色法行SLNB,对SLN进行HE染色、细胞角蛋白19(CK19)的免疫组化染色(IHC)及RT-PCR检测,比较3种方法对SLN微转移灶检出的敏感性、准确率的差异。结果21例中,成功检出SLN 16例,检出率为76.2%。IHC、RT-PCR与HE比较差异有统计学意义。结论前哨淋巴结活检可有效判断甲状腺癌转移状态,应用RT-PCR检测CK19在SLN中的表达,可提高敏感度及准确率。
Objective To investigate the possibility of sentinel lymph node biopsy （SLNB） in thyroid carcinoma and value of reverse transcripase chain reaction（RT-PCR） in judging micrometastasis in sentinel lympy node（SLN）. Methods Twenty - one patients with thyroid carcinoma were injected methylene blue to locate the SLN. The SLN were examined with HE staining, IHC examined of cytokinel9（CK19） and RT- PCR. The accuracy, sensitivity of HE staining,IHC examination and RT- PCR were compared. Results SLN were successfully identified in 16（76.2% ） of 21 patients. IHC and RT - PCR had obvious discrepancy with HE staining in the accuracy, sensitivity. Conclusion SLNB is useful to determine the status of lymph nodes of thyroid carcinoma. CK19 RT - PCR can improve the sensitivity and accuracy of micrometastases in SLN.
出处
《临床医学》
CAS
2008年第11期98-99,共2页
Clinical Medicine
基金
广州市医药卫生科技项目(编号:2006-YB-025)
关键词
甲状腺肿瘤
前哨淋巴结
角蛋白19
微转移
Thyroid neoplasms
Sentinel lymph node
Cytokeratin 19
Micrometastases
