摘要
目的:阐明炎症肠道疾病(inlammatory bowel disease,IBD)中肺表面活化蛋白A(pulmonary surfactant protein A,SP-A)相似分子的分布特征和CD68阳性巨噬细胞的免疫反应。方法:外科手术获得的结肠组织标本来自布朗大学医学院罗得岛医院病理科,应用免疫组织化学方法检测IBD患者肠道组织中SP-A相似分子的表达。结果:SP-A相似分子分布在肠道上皮、肠道绒毛表面,连接组织血管和某些炎症细胞。具有SP-A相似分子和CD68阳性表达的巨噬细胞数量炎症区域明显增加,高于正常组织。而免疫荧光双标记实验显示,一些CD68阳性巨噬细胞可以表达SP-A相似分子免疫反应。结论:SP-A是肺脏一种重要的宿主防御因子。
Objective To investigate the distribution of pulmonary surfactant protein A （ SPA ） like molecules and the bridge of frontier host defense and adaptive immune response cell of CD68 positive macrophages in inflammatory bowel disease （IBD）. Methods Surgical specimens derived from involved areas and normal area of the colon with Crohn disease （CD） and ulcerative colitis （UC） were obtained from Department of Pathology, Rhode Island Hospital, Brown University Medical Center. The distribution of SP-A like molecule in intestine of IBD was detected by immunohistochemistry. Results SP-A like molecule located in epithelia of intestine, the surface of intestine viii, blood vessels of connective tissue, and some inflammatory cells. The number of macro-phages with both SP-A like molecule and CD68 positive was dramatically increased in the inflammatory area than the normal area. Some CD68 positive macrophages expressed SP-A like immunoreactivity by immunofluorescence double labeling. Conclusion SP-A is an important host defense molecule in lung, and SP-A expression in large intestine may reflect a close relation between 2 organs in immune response towards inflammation.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2008年第11期979-986,共8页
Journal of Central South University ：Medical Science
