摘要
目的了解烟台地区多重耐药铜绿假单胞菌16SrRNA甲基化酶、氨基糖苷类修饰酶基因的存在状况。方法自2006年1-6月间烟台地区住院病人标本中分离出30株多重耐药的铜绿假单胞菌,(K-B)法测定16种抗菌药物的敏感性;PCR法检测16SrRNA甲基化酶、氨基糖苷类修饰酶基因。结果30株多重耐药铜绿假单胞菌中16SrRNA甲基化酶(armA、rmtA、rmtB、rmtC、rmtD、rpmA)基因均为阴性;氨基糖苷类修饰酶aac(3)-Ⅱ基因阳性株6株、aac(6′)-Ⅱ基因阳性株14株、ant(3″)-Ⅰ基因阳性株11株、ant(2″)-Ⅰ基因阳性株1株;aac(3)-Ⅰ和aac(6′)-Ⅰb基因均为阴性。结论烟台地区多重耐药铜绿假单胞菌对氨基糖苷类药物耐药的主要原因与aac(3)-Ⅱ、aac(6’)-Ⅱ、ant(3")-Ⅰ和ant(2")-Ⅰ4种氨基糖苷类修饰酶基因存在有关。尚未检出16SrRNA甲基化酶。
Objective To survey the prevalence of genes encoding 16SrRNA methylase and aminoglycoside modifying enzyme in multi - resistant Pseudomonas aeruginesa . Methods The samples of 30 multi - resistant Pseudomonas aeruginosa isolates were collected from inpatients from January 2006 to June 2006 in Yantai. The sensitivity of the isolates to 16 antibacterial agents was determined using paper diffusing method. The genes of 16S rRNA methylase and aminoglycoside modifying enzyme were analyzed by polymerase chain reaction . Results 16S rRNA methylase gene was identified in none of the 30 isolate. Aminoglyceside modifying enzymegene aac （3） - Ⅱ gene was fond in 6 isolates , aac （6＇） - Ⅱ were identified in 14 isolates, ant （3＂） - Ⅰ in 11 isolates , and ant （2＂） - Ⅰ in 1 isolate; aae （3） - Ⅰ and sac （6＇） - Ⅰ were negative. Conclusion Multi - resistant Pseudomunas aeruglnosa was resistant to aminoglycoside mainly due to the presence of aminoglycoside mainly enzyme sac （3） - Ⅱ, sac （6＇） - Ⅱ, ant （3＂） - Ⅰ , ant （2 ＂） - Ⅰ in Yantai region. 16S rRNA methylase were not detected.
出处
《医学动物防制》
2008年第12期885-887,F0003,共4页
Journal of Medical Pest Control
基金
山东省技术发展攻关项目
编号:2008GG10002040
关键词
铜绿假单胞菌
甲基化酶
氨基糖苷类修饰酶
耐药性
Pseudomonas aeruginosa
Methylase
Aminoglycoside modifying enzyme
Mul- tidrug resistance
