摘要
目的探索一种新的脱细胞阴茎海绵体基质的制备方法。方法取健康壮年兔完整阴茎海绵体组织,以Triton-X100与NH3.H2O(氨水)联合提取法进行脱细胞处理。标本作HE染色,组织学观察分析脱细胞效果。结果脱细胞处理25天后,成功获得脱细胞海绵体基质。所得基质外观良好。HE染色观察无细胞存在,弹力纤维排列规整,间隙较大,结构无破坏。结论利用Triton-X100与NH3.H2O联合提取法可成功制备完整无细胞阴茎海绵体基质。
Objective To find a new method to prepare the acellular matrix of the penile cavernous body. Methods Intact specimens of the penile cavernous body were collected from healty adult rabbits, treated with Triton-X100 and NH3 · H2O, and then stained with HE. Light microscopy was employed to observe the acellular ceils. Results Acellular matrix of the penile cavernous body was obtained after a 25-day treatment with Triton-X100 and NH3 · H2O. The matrix was intact. HE staining showed that the matrix was composed of regularly arranged elastic fibers with wide intra-spaces without any ceils inside. Conclusion Acellular matrix of the penile cavernous body can be prepared by using Triton-X100 and NH3 · H2O.
出处
《中国微创外科杂志》
CSCD
2008年第12期1137-1139,共3页
Chinese Journal of Minimally Invasive Surgery
基金
深圳市科技局重点基金资助项目(JH200505270416B)
广东省医学科研基金资助项目(A2005646)
