摘要
目的研究人参皂甙对去势大鼠阴茎海绵体组织细胞凋亡、NO含量的影响,探讨人参皂甙壮阳功效的可能机制。方法40只成年雄性大鼠随机分为去势组、对照组及不同剂量(25mg/kg、100mg/kg)人参皂甙组共4组,1周后取阴茎海绵体,放免法检测血清睾酮含量(ng/ml),全自动生化分析仪比色法测定海绵体NO含量(μg/g),末端脱氧核糖核酸转移酶介导的duTP缺口末端标记法测定细胞凋亡。结果对照组血清睾酮水平浓度为(1.51±0.86),在去势组、人参皂甙治疗组(25mg/kg、100/mg/kg)均未测到。去势组阴茎海绵体NO浓度(14.45±2.38)较对照组(39.8±3.28)显著降低(P<0.01),25mg/kg人参皂甙组阴茎海绵体NO水平(16.02±2.67)与去势组(14.45±2.38)接近(P>0.05),100mg/kg人参皂甙组NO水平(37.88±7.06)较去势组细胞凋亡数(14.45±2.38)明显升高(P<0.05),与对照组(39.8±3.28)接近(P>0.05)。大剂量100mg/kg人参皂甙组(12.51±1.81)较去势组(26.02±5.25)低(P<0.05)。25mg/kg人参皂甙组凋亡细胞积分光密度(27269.60±4920.42)与去势组比较(33931.50±2459.36)差异无统计学意义,大剂量100mg/kg人参皂甙组(18766.36±3040.42)较去势组(33931.54±2459.36)低,两者比较差异有统计学意义(P<0.05)。结论100mg/kg剂量的人参皂甙不能增加去势大鼠血清睾酮含量,但可以提高去势大鼠阴茎海绵体组织NO水平,减少海绵体细胞凋亡。人参皂甙对去势大鼠阴茎海绵体细胞凋亡的抑制作用可能与其增加NO水平有关。
Objective To study the effect of ginsenoside on apoptosis, NO concentration during castration-induced rat corpus cauernosum cells. Methods Forty mature male, rats were randomly divided into 4 groups： one-week castrated, one-week, castrated with two ginsenosides groups： （25mg/kg, 100mg/kg ） at times of surgury and sham-operated （control）. each group contains 10 animals. One week after castration, corpus cavernosum were harvested. Plasma testosterone and, NO concentration in rats corpus cavemosum were measured. Apoptosis was detected by using in situ Terminal Dexynucleotidy Transferase-mediated duTP nick end labeling（TUNEL）and quantitated. Results Plasma testosterone level in the control group was （1.51±0.86）, other group was not detected, compared to cortrol group（39.8±3.28）, NO concentration in RCC from the castration group （14.45±2.38） was greatly decreased, compared to the castration group （14.45±2.38）, NO concentration in RCC from 100mg/kg ginsenoside group （37.88±7.06） was greatly increased （P〈0.05）; Compared to castration group （26.02±5.25） apoptosis extent of RCC was supressed from 100 mg/kg G.S group （12.51±1.81） （P〈0.05）. Conclusion Ginsonoside does not contain hormone like components and does not increase the plasma testosterone level on castrated rat. Castration can induce apoptosis in RCC which could be prevented by large dose of ginsenoside （100 mg/kg） and this effect could be attributed to its action of the activation of NO pathway.
出处
《中国男科学杂志》
CAS
CSCD
2007年第11期15-19,共5页
Chinese Journal of Andrology
