摘要
目的:探讨热放疗对舌鳞癌Tca8113细胞和耐药的Tca8113/CBDEA细胞耐药性的影响。方法:实时定量逆转录PCR(RT-PCR)检测热放疗对Tca8113细胞和耐药的Tca8113/CBDEA细胞多药耐药基因1(MDR1)、多药耐药相关蛋白1基因(MRP1)和谷胱甘肽硫-转移酶π基因(GST-π)表达的影响和检测热放疗对细胞内阿霉素浓度的影响。结果:Tca8113/CBDEA和Tca8113细胞热放疗后耐药基因MDR1的表达在4h和24h无明显改变(P>0.05)。MRP1基因的表达在4h和24h组均有明显下降(P<0.05),GST-π基因表达在4h组Tca8113和Tca8113/CBDEA的表达无明显改变(P>0.05),在24h组有明显下降(P<0.05).热放疗以后肿瘤细胞内阿霉素(ADM)浓度有明显上升。结论:热放疗联合应用抑制了放疗造成的耐药基因表达上升,增加了细胞内的药物浓度,热放疗联用不会促使肿瘤细胞产生MDR。
Objective: To study effect of thermoradiotherapy on expressions of MDR genes in tongue squamous cell carcinoma cell line Tca 8113 and its muhidrug resistance (MDR) cell line Tca8113 / CBDEA and intracellular ADM concentration.Method: Cell lines were thermoradiotherapy -treated (42 ℃ for 0.5 h and 2Gy of radiotion), 4 h and 24 h later real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) detected expressions of MDR relative genes (MRP1, MRP1, GST-π) and HTS 7000 Plus Bio Assay Reader measured intraeellular ADM concentration.Result: Post thermoradiotherapy, detection of real time PCR showed there were not alteration in the expression of MDR1 gene (P〉0.05) .Expressions of MRP1 gene significantly descended at 4 h and 24 h postthermoradiotherapy (P〈0.05).PCR showed there were not alteration in the expressions of GST-π gene at 4h postthermoradiotherapy in Tca 8113 and Tca 8113 / CBDEA cell line (P〉0.05), but there was significantly decrease at 24 h (P〈0.05). Drug tolerance decreased.hyperthermia increased intraeellular drug concentration in Tca 8113 / CBDEA and Tca 8113 (P〈0.01).Conclusion: thermoradiotherapy enhance chemotherapy effect and suppress the expressions of MDR genes induced by radiation.The combination of hyperthermia and radiotherapy does not induce MDR.
出处
《临床口腔医学杂志》
2008年第11期670-673,共4页
Journal of Clinical Stomatology
基金
国家自然科学基金资助(39970798)
湖北省十堰市科技基金资助
关键词
舌鳞状细胞癌
多药耐药
热放疗
化疗
tongue squamous cell carcinoma
muhidrug resistance
hyperthermia