摘要
目的探讨热疗对舌鳞癌Tca8113细胞和耐药的Tca8113/CBDEA细胞耐药性的影响。方法采用实时定量逆转录PC(RRT-PCR)检测42℃,0.5h热疗对Tca8113细胞和耐药的Tca8113/CBDEA细胞多药耐药基因(1MDR1)、多药耐药相关蛋白1基因(MRP1)和谷胱甘肽硫-转移酶π基因(GST-π)表达的影响以及检测热疗对细胞内阿霉素浓度的影响。结果Tca8113/CBDEA细胞在热疗后4h和24hMDR1、MRP1、GST-π耐药基因表达量明显下降(P<0.01),Tca8113细胞的MDR1、MRP1耐药基因表达在4h和24h时亦有明显下降(P<0.05),GST-π在24h有明显下降(P<0.01)。热疗以后肿瘤细胞内阿霉素(ADM)浓度有明显上升(P<0.01)。结论热疗抑制了耐药基因的表达,增加了细胞内的药物浓度,热疗可能是逆转肿瘤细胞耐药,提高化疗效果的一种有效手段。
Objective To study the effect of hyperthermia on expressions of multidrug resistance （MDR） genes in tongue squamous cell carcinoma cell line Tca8113 and its MDR cell line Tca8113/CBDEA and intracellular ADM concentration. Methods Cell lines were hyperthermia-treated（42℃ for 0.5 h）, 4 h and 24 h later real-time quantitative reverse transcriptase-polymerase chain reaction （RT-PCR） detected expressions of MDR relative genes （MDR1, MRP1, GST-π）and HTS 7000 Plus Bio Assay Reader measured intracellular ADM concentration. Results Expressions of MDR1, MRP1, GST-π genes in Tca8113/CBDEA significantly descended at 4 h and 24 h post-hyperthermia （P〈0.01）. Expressions of MDR,, MRP, genes in Tca8113 significantly descended at 4 h and 24 h post-hyperthermia （P〈0.05）, but there was not statistical difference between 4 h and 24 h post-hyperthermia. GST-π expression had not statistical descent at 4 h, but there was significantly descend at 24 h post-hyperthermia （P〈0.01）. Drug tolerance decreased and hyperthermia increased intraeellular drug concentration in Tea8113/CBDEA and Tea8113 （P〈0.01）. Conclusion Hyperthermia enhance chemotherapy effect and reverse MDR genes expression in tongue squamous cell carcinoma cell line. It is suggested that hyperthermia could be used as a method to overcome MDR, which is worth more investigation.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2006年第5期447-450,共4页
West China Journal of Stomatology
基金
国家自然科学基金资助项目(39970798)
四川大学博士研究基金资助项目(0040305505010)
关键词
舌鳞状细胞癌
多药耐药
热疗
tongue squamous cell carcinoma
multidrug resistance
hyperthermia
