摘要
目的：建立乳腺癌骨转移动物模型。方法：40只4周~6周龄雌性裸鼠随机分为5组,每组8只,用浓度分别为1×105/ml、1×106/ml、5×106/ml、1×107/ml、5×107/ml的MDA-MB-231细胞悬液0.1ml行左心室注射。观察裸鼠左心室注射致死情况及存活裸鼠49 d后骨转移发生情况。结果：左心室穿刺注射总致死率为32.50%（13/40）;各组左心室穿刺注射致死率分别为1/8、1/8、1/8、3/8、7/8,组间比较差异有统计学意义（P=0.004）。全组骨转移发生率为48.15%（13/27）;各组骨转移发生率分别为0/7、3/7、5/7、4/5、1/1,各组骨转移发生情况差异有统计学意义（P=0.014）。结论：经裸鼠左心室穿刺注射肿瘤细胞成功建立了乳腺癌骨转移动物模型,肿瘤细胞浓度为5×106/ml时是经裸鼠左心室注射建立乳腺癌骨转移动物模型的适宜浓度。
Objective ： To establish an animal model of human breast cancer metastasis to bone in vivo. Methods： Forty female nude mice aged 4 -6 weeks were divided equally into 5 groups, and injected with MDA-MB-231 cells suspended in 0.1 ml PBS at the concentrations of 1 × 10^5 （ group 1 ）, 1 × 10^6 （ group 2）, 5 × 10^6（ group 3）, 1 × 10^7（ group 4） and 5 × 10^7/ml （group 5） into the left heart ventricles. Deaths following the injection were recorded, and the mice that survived 49 days after the injection were subjected to pathological examination to determine bone metastasis. Results ： Thirteen （32.50%） of 40 mice died after the injection, 1/8, 1/8, 1/8, 3/8 and 7/8 respectively in the 5 groups, with statistically significant differences in mortality （P = 0.004）. The total bone metastasis rate was 48.15% （13/27）, 0/7, 3/7, 5/7, 4/5 and 1/1 respectively in the 5 groups, with significant differences in between （P = 0. 014）. Conclusion ： We successfully established an animal model of breast cancer bone metastasis in vivo by injecting MDA-MB-231 cells at different concentrations into the left heart ventricles of nude mice, and found that the suitable concentration was 5 × 10^6 MDA-MB-231 cells suspended in 0.1 ml PBS.
出处
《医学研究生学报》
CAS
2008年第11期1143-1145,I0016,共4页
Journal of Medical Postgraduates
基金
国家自然科学基金资助项目(批准号:30700814)
第三军医大学2006年度中青年科研基金资助项目
关键词
乳腺癌
骨转移
动物模型
左心室注射
MDA-MB-231细胞株
Breast cancer
Bone metastasis
Animal model
Injection into the left cardiac ventri- cle
MDA-MB-231 cell line
