摘要
目的对三种乳腺癌骨转移模型复制及其检测方法进行比较研究.方法选择BALB/cA-nu/nu雌性6-8周龄裸鼠24只,分为原位注射组、尾静脉注射组和左心室注射组3组,每组8只,分别注射0.2 mL人乳腺癌MDA-MB-435细胞悬液(2×107/mL);40 d后做X-ray、PET-CT检查;45 d后进行动物解剖,取影像学阳性部位送病理检查.结果PET-CT观察到骨侵蚀影像;肉眼见明显的肋骨连续性中断;病理检查结果显示胸廓肋骨部位有肿瘤细胞浸润;X-ray检查未发现明显影像学改变.左心室注射法骨转移率为75%,尾静脉注射法骨转移率为12.5%,原位注射法未见骨转移.结论左心室注射法复制乳腺癌骨转移模型,与另外两种方法相比,可以获得较高骨转移率,是复制乳腺癌骨转移模型较为理想的一种方法.
Objective o investigate three methods for replicating the animal model of bone metastasis from breast cancer and the detections of bone metastasis. Methods Twenty - four BALB/cA - nu/nu female nude mice were divided into three groups, and each mouse was injected with 0. 2 mL MDA - MB -435 cells suspension （2 × 10^7/mL） into the body via mammary gland, tail vein and the left ventricle of heart respectively according to the experiment design. Forty days later, X - ray and PET - CT were applied to detect lesions in bones. All mice were dissected forty - five days later and the positive tissue/organ in imageology or nude eye were confirmed in pathology. Results Significant osseous lesions were detected by PET - CT, however, that lesions didn＇t show up in X - ray image. The results of pathology indicated that the metastatic cells also were found in thoracic cage and the ribs. The rate of bone metastases of left ventricle injection group was 75% and that of the tail vein injection group wasl2.5%. And no bone metastasis was found in the mammary gland group. Conclusion Compared to these methods, injection via the left ventricle of heart can attain a higher bone metastasis rate and play an important role in replicating animal model of bone metastasis from breast cancer.
出处
《昆明医学院学报》
2007年第1期14-18,共5页
Journal of Kunming Medical College
基金
国家自然科学基金资助(30460142)
昆明医学院群体创新基金资助(KMC2005DG01)
关键词
乳腺癌
骨转移
模型
Breast cancer
Bone metastasis
Model
