摘要
采用PCR-SSCP方法对186头24月龄秦川牛IGF2基因进行了SNPs多态性检测,并将其与部分胴体和肉质性状进行关联分析。在IGF2基因120碱基处发现C→T突变,在279碱基处发现A→G突变。方差分析结果表明:BB、DD两个位点与胴体性状中与宰前活重、胴体重、胴体长、胴体胸深、眼肌面积显著相关(P<0.05),其中背部皮下脂肪厚达到差异极显著(P<0.01);与肉质性状大理石花纹、嫩度、pH24(牛肉排酸24h后的酸度值)显著相关(P<0.05)。但是在胴体深、系水力指标中差异不显著(P>0.05)。A、D等位基因是群体中的优势等位基因,AA、DD基因型是优势基因型,而含有B、D等位基因的个体的胴体和肉质性状优于其他个体。
PCR-SSCP technology was used to analyze the correlation of polymorphisms IGF2 （Insulin-like growth factor ） gene with carcass and meat quality traits in 186 Qinchuan cattle at the age of 24-month. C→T mutation in 120 and A→G mutation in 279 of IGF2 gene. Statistical analysis indicated that Qinchuan cattle with genotype BB and DD had significant differences in slaughter weight, carcass weight, carcass length, carcass chest depth and eye muscle area （P〈0.05）. The dif- ference in thickness of back fat was significant（P〈0.01）. Significant differences in marbling, tenderness, pH24 of meat qual- ity（P〈0.05）, but the differences in carcass depth and water holding capacity were not significant （P〉0.05）. Genotype AA, DD were predominant genotypes and A, D were predominant alleles. The population containing B, D allele had more excellent carcass and meat quality than others, especially in the capacity of fat accumulate
出处
《遗传》
CAS
CSCD
北大核心
2008年第12期1579-1584,共6页
Hereditas（Beijing)
基金
国家高技术研究发展计划项目(863计划)(编号:2006AA10Z1A1)
陕西省2006年重大科技专项(编号:2006KZ07-G1)
西北农林科技大学拔尖人才支持计划基金资助~~
