摘要
以阴离子型层状材料纳米晶镁铝水滑石为载体通过直接吸附对脂肪酶进行固定,考察了各因素对酶固定化的影响,优化了固定化条件。研究表明,脂肪酶的较优固定化条件为载体用量0.32 g/mL(720 U/mL 酶液),30～35℃,pH 值7.5,负载6～7 b,制得的固定化酶表观酶活达725 U/g。游离酶和固定化酶的水解活化能分别为5.45 kJ/mol 和16.31 kJ/mol,游离酶和固定化酶的表观失活活化能分别为20.28 kJ/mol 和29.02 kJ/mol,固定化酶较游离酶稳定。
A method of immobilizing lipase was described anion nano-crystalline Mg-Al hydrotalcite with layer-shape and double hydroxides was used as a adsorbing carrier. The influence of several factors on the lipase immobilization was studied. The optimal conditions were as follows- carrier dosage 0.32 g/mL （720 U/mL enzyme solutions）, pH 7.5 and incubating time 6-7 h at 30-35 ℃. The apparent activity of the immobilized lipase was 725 U/g. The apparent inactivation energy of free and immobilized lipase were 20.28 and 29.02 kJ/mol, respectively, so the stability of the immobilized lipase was higher than that of free lipase.
出处
《化学反应工程与工艺》
EI
CAS
CSCD
北大核心
2008年第5期455-460,共6页
Chemical Reaction Engineering and Technology
基金
国家863项目(2003AA214061)
关键词
酶固定化
脂肪酶
镁铝水滑石
载体
enzyme immobilization
lipase
magnesium-aluminium hydrotalcite
carrier
