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尖叶走灯藓(Plagiomnium cuspidatum)叶绿素荧光对复合重金属胁迫的响应 被引量:45

Physiological response of chlorophyll fluorescence in moss Plagiomnium cuspidatum to mixture heavy metal solution
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摘要 利用快速叶绿素荧光动力学技术研究了Cu2+、Zn2+、Cd2+、Pb2+四种重金属离子复合污染下尖叶走灯藓Plagiomnium cuspidatum(Hedw.)T.Kop.叶绿素荧光动力学以及叶绿素含量的变化。结果显示,重金属胁迫导致尖叶走灯藓PSII反应中心的最大光化学效率(Fv/Fm)、光合机构电子传递的量子产额(ETo/ABS)、捕获的激子将电子传递到电子传递链中超过QA的其它电子受体的概率(ETo/TRo)、单位叶面积的反应中心的数量(RC/CSo)降低。重金属胁迫也显著降低尖叶走灯藓植物体叶绿素a、叶绿素b、总叶绿素含量以及Chla/b的值,降低的程度与重金属浓度和胁迫时间有关。尖叶走灯藓可以耐受10μmol/L浓度的复合重金属污染,在10μmol/L浓度范围内,尖叶走灯藓PSII最大光化学效率Fv/Fm、单位面积有活性反应中心的数目RC/CSO、捕获的激子将电子传递到超过QA的其它电子受体的概率ETO/TRO以及用于电子传递的量子产额ETO/ABS等与对照相比均无明显变化,表明在此浓度范围内,重金属不会对尖叶走灯藓的光合器官造成伤害。50μmol/L和100μmol/L浓度的重金属则造成光合系统的不可逆损伤。 Chlorophyll-fluorescence were measured by a handy PEA fluorometer in moss Plagiomnium cuspidatum ( Hedw. ) T. Kop. in response to a mixture heavy metal cations of Cu^2+ ,Zn^2+ , Cd^2+ and Pb^2+at concentrations of 0, 1, 5, 10, 50 and 100μmol/L respectively. Exposure in a mixture heavy metal solution 10-day period resulted in a decline of chlorophyll fluorescence and content of chlorophyll. Heavy metals used within 10μmol/L, a little change of all the analyzed values of Fv/Fm,ETo/ABS,ETo/TRo and RC/CSo was observed compare with controls, but a dramatic decrease occurred when the metal concentration increased to 50 and 100μmol/L. The result indicated that the photosynthetic apparatus of the moss Plagiomnium cuspidatum was not badly damaged when the mixed-heavy metal solution became lower than 10μmol/L concentrations, but the damage was not reversible up to 50 or 100μmoL/L.
出处 《生态学报》 CAS CSCD 北大核心 2008年第11期5437-5444,共8页 Acta Ecologica Sinica
基金 广东省农业环境综合治理重点实验室开放课题资助项目 山东省自然科学基金资助项目(Y2005D08) 青岛农业大学人才基金资助项目(630744)~~
关键词 尖叶走灯藓 重金属 叶绿素荧光 叶绿素含量 Plagiomnium cuspidatum ( Hedw. ) T. Kop. heavy metal chlorophyll fluorescence content of chlorophyll
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