摘要
目的:分析游离胰岛素样生长因子(FIGF)-Ⅱ异常表达对肝癌(HCC)的诊断和鉴别价值。方法:收集慢性肝病患者外周血,以酶联免疫吸附试验法(ELISA)测定肝癌(146例)、肝硬化(25例)、慢性肝炎(30例)、非肝肿瘤患者(19例)和正常对照者(30例)的血TGF-β1和FIGF-Ⅱ水平,分析两者在HCC早期诊断和鉴别方面的临床价值。结果:HCC患者血FIGF-Ⅱ呈异常表达水平,显著高于正常组、肝硬化、慢性肝炎和非肝肿瘤组(P<0.05)。如以FIGF-Ⅱ浓度>6.0μg/L为界,肝癌诊断的灵敏度为76.0%,特异性为79.7%。与血AFP浓度、肿块大小均无相关,与AFP联合检测可提高HCC诊断阳性率。结论:血FIGF-Ⅱ的表达异常与肝癌形成有关,两者的检测有助于肝癌的诊断和鉴别。
Objective ：To investigate the clinical values of circulating free IGF-Ⅱ（FIGF-Ⅱ） expression in diagnosis of hepatocellular carcinoma （HCC）.Methods：The levels of circulating FIGF-Ⅱ expression were analyzed with enzyme-linked immunosorbent assay （ELISA） in 146 HCC patients, 25 liver cirrhosis（LC） patients, 30 chronic hepatitis（CH） patients, 19 other gastroenterie cancer patients, and 30 health controLs.The clinical significance was investigated in diagnosis and differentiation of HCC. Results：The leveLs of FIGF- Ⅱ in HCC patients were significantly higher than those in normal subjects,LC,CH, and other gastroenteric cancers （P〈0.05） respectively. The sensitivity and specificity of its assay were 76% and 79.7% in FIGF- Ⅱ level for HCC diagnosis with over 6.0 μg/L of FIGF- Ⅱ level as normal up-limitation respectively. No significant correlation was found between FIGF- Ⅱ levels in HCC patients and AFP levels of the tumor sizes. Combining analysis of two markers and AFP could increase the detection frequencies for HCC diagnosis. Conclusion：The abnormal expressions of circulating FIGF-Ⅱ are probably correlated with the development of HCC,and its analysis is helpful to diagnose HCC and differentiation.
出处
《现代医药卫生》
2008年第24期3654-3656,共3页
Journal of Modern Medicine & Health
基金
江苏省卫生科技项目(H200523)部分资助
