摘要
目的探讨载脂蛋白B(apolipoprotein B,ApoB)基因-516C/T多态性与冠心病易感性的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测162例冠状动脉粥样硬化性心脏病(简称冠心病)患者(冠心病组)和186名对照者(对照组)的ApoB基因-516C/T基因型和等位基因的分布,并进行对比分析及相关性分析。结果基因型鉴定结果显示,两组均存在CC型、TT型及CT型基因;冠心病组ApoB基因T等位基因和TT基因型的频率(分别为29.01%、12.96%)均明显高于对照组(19.35%、4.84%),差异均有统计学意义(P<0.05或0.01);回归分析显示,T等位基因携带者较非携带者罹患冠心病的危险性显著增加(P<0.01)。此外,对照组中T等位基因携带者血清总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)水平均明显高于非携带者(均P<0.05)。结论ApoB基因-516C/T多态性与冠心病的易感性相关,T等位基因可能是冠心病发病的一个危险的遗传因素。
Objective TO investigate the association of apolipoprotein B （ApoB） gene -516C/T polymorphism with coronary heart disease （CHD） in Chinese Han Population. Methods ApoB gene -516C/T polymorphism was measured using polymerase chain reaction-restrictive fragment length polymorphism （PCR-RFLP） in 162 CHD patients and 186 controls, the contrastive analysis and the relevant analysis of genotype and allele distribution was performed. Results Distribution of ApoB genotype was in Hardy-Weinberg equilibrium in control group. The frequency of -iT genotype in CHD group was significantly higher than that in control （12.96% vs 4.84% ,P〈0.05）; and the frequency of T allele in CHD group was significantly higher than that in control （29.01% vs19.35%, P〈0.01） .After adjusted for age ,gender and other CHD risk factors by multiple Logistic regression analysis, the odds ratio of T allele carriers for coronary heart disease was increased than that of non-careers（OR =1.92; 95% CI =1.16-3.18）. Furthermore, Ptasma TC and LDL-C levels in T allele carriers were markedly higher than non-T allele carriers in controls （P〈 0.05）. Conclusion ApoB gene -516C/T polymorphism is associated with CHD risk in Chinese Hart population, and T allele may serve as genetic determinants for CHD.
出处
《浙江医学》
CAS
2008年第10期1046-1048,共3页
Zhejiang Medical Journal
关键词
载脂蛋白B
基因多态性
冠心病
Apolipo protein B Coronary heart disease Gene polymorphism
