摘要
目的:研究康定地区人工种植掌叶大黄的高效液相色谱(HPLC)指纹图谱。方法:色谱柱为Shim-packVP-ODS(150mm×4.6mm,5μm),流动相为0.1%磷酸甲醇-0.1%磷酸水溶液(梯度洗脱),流速为1.0mL·min-1,柱温为30℃,检测波长为280nm。结果:建立了康定地区人工种植掌叶大黄的HPLC指纹图谱,确定了56个共有峰,并且对10批药材进行了相似度计算,建立了共有模板。结论:本方法简单可行,能够从整体上反映康定地区人工种植掌叶大黄的质量。
OBJECTIVE： To study the HPLC fingerprints of Rheum palmatum planted in Kangding area. METHODS： The samples were separated on a Shim - pack ODS（ 150 mm× 4.6 mm, 5μm） with column temperature kept at 30 ℃ ; the mobile phase consisted of 0.1% H3PO4 methanol - 0.1% HaPO4 aqueous solution by linear gradient elution with a flow rate of 1.0 mL · min^- 1 and detection wavelength of 280 nm.RESULTS：The HPLC fingerprints of Rheum palmatum planted in Kangding area had been established in which 56 common peaks were noted. Similarity of ten batches crude medicines was calculated, and a common template was set up. CONCLUSION ：The established method is simple and feasible and by which the general quality of Rheum palmatum planted in Kangding area can be reflected.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第33期2590-2592,共3页
China Pharmacy
