摘要
目的观察不同剂量的20(S)-原人参二醇(Protopanaxadiol,PPD)在体内外对人肝癌细胞株SMMC-7721抗肿瘤作用。方法建立人肝癌裸鼠皮下移植瘤模型,观察20(S)-原人参二醇的肿瘤抑制作用。MTT比色法检测20(S)-原人参二醇对SMMC-7721细胞的增殖抑制作用,Ho-echst33342核染色观察细胞凋亡形态学改变,采用FITC-An-nexinⅤ/PI双染流式细胞术分析凋亡情况,同时检测Caspase-3活性。结果在体内,PPD可抑制SMMC-7721细胞裸鼠异种移植瘤生长;在体外,PPD对SMMC-7721细胞的增殖具有明显的抑制及诱导其凋亡作用,呈时间和剂量依赖性,Hoechst33342核染色可见凋亡小体,同时伴有Caspase-3活性的增加。结论20(S)-原人参二醇在体内外均可抑制SMMC-7721细胞增殖,并诱导其凋亡,其机制可能通过活化Caspase-3诱导细胞凋亡而发挥抗肿瘤作用。
Aim To study the anti-tumor effect of 20 （S） -Protopanaxadiol （PPD） at different concentration on human liver cell line SMMC-7721 in vivo and in vitro. Methods The subcutaneous transplantable tumor model of human liver cancer in nude mice was established and the anti-tumor effect was calculated. Cell growth rate was determined with MTF assay. The apoptosis was analyzed by FITC-Annexin V/PI and Hoechst33342 staining method, and the activity of Caspase-3 was detected. Results In vivo, PPD could obviously inhibit the growth of transplanted tumor. Invitro, PPD induced inhibition of human liver cancer SMMC-7721 cells was time-dependent and dose-de- pendent. The apoptotic body was observed by Hoechst33342 staining. PPD could induce cell apoptosis of SMMC-7721 ,and the increase of Caspase-3 activity was observed in each PPD group. Conclusion PPD could inhibit the growth of human liver cancer SMMC-7721 cells in vivo and in vitro by up-regulating the activity of Caspase-3 and inducing the cell apoptosis.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2008年第11期1504-1508,共5页
Chinese Pharmacological Bulletin
基金
国家"九五"重点科技攻关课题(No96-901-01-83)
