摘要
以纯水为流动相,用PL aquagel-OH40色谱柱与自动馏分收集器,在Agilent1100高效液相色谱仪上,对巯基丁二酸(MSA)单层包裹的粒径约1-5nm的水溶性小金纳米簇进行尺寸排阻色谱(SEC)分离,环境友好,操作简便,重现性好.TEM表征和粒径统计分析表明:在不同洗脱时间收集的金纳米簇馏分与相应的原始样品相比其粒径分布均得到改善,尺寸分布明显窄化.金纳米簇在所用色谱柱上的分离主要按尺寸排阻机制进行.
Using pure water as a mobile phase, water-soluble small gold nanoclusters capped by 2-mercaptosuccinic acid （MSA） monolayer with the size range of 1-5 nm are separated by size exclusion chromatography （SEC） with PL aquagel-OH 40 column and au- tomatic fraction collector on Agilent1100 HPLC. It is environment-friendly and easy to operate. The reproducibility is good. The results of TEM characterization and particle size statistics show that the monodispersity of the gold nanoclusters in the collected fractions at different elution times is improved as compared to that of the original samples, and obviously the particle size distribution is narrowed. The separation of gold nanoclusters on the chromatography column mainly follows a SEC mechanism.
出处
《四川师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第6期757-761,共5页
Journal of Sichuan Normal University（Natural Science）
基金
国家自然科学基金(20473004)资助项目
关键词
水溶性金纳米簇
尺寸排阻色谱
分离
Water-soluble gold nanoclusters
Size exclusion chromatography
Separation
