摘要
目的探讨甲胎蛋白异质体（AFP-L3）的检测在预警原发性肝癌中的作用。方法对100例AFP升高肝病患者血清，应用甲胎异质体微量离心柱分离并洗脱获得AFP-L3，再同时检测原始血清中的AFP以及AFP-L3含量，计算AFP-L3在AFP中的比例，对AFP-L3异常升高者、正常者进行跟踪，结合6个月后临床诊断结果，分析AFP-13升高在鉴别良性肝脏病变与预警肝癌中的作用。结果肝癌、疑似肝癌患者与良性肝病患者中的AFP-L3阳性率差异有统计学意义（分别为81．80％、73．68％、11．80％，P〈0．05）。未确诊肝癌（疑似HCC、肝病）的患者中，AFP—L3阳性的21例中有12例在6个月内被诊断为HCC，而且有6例是通过B超、CT等影像学手段被早期确诊的单发性小肝癌。AFP-L3阴性的62例标本中，6个月内有3例发生肝癌，AFP-L3阳性发生肝癌的危险率增加了11．9倍。结论AFP-L3与AFP值无相关性，可以作为一个独立肝细胞癌诊断因子。AFP-L3的测定对于AFP升高时良、恶性肝病的鉴别及肝癌的早期预警诊断具有重要意义。
Objective To explore the significance of Lens cnlinafis-reactive α-Fetoprotein （AFP-I.3） detection in primary hepatocellular carcinoma. Methods AFP-L3 was isolated by using microspincolumn coupled with lens culinaris agglutinin（LCA）, AFP and AFP-L3 were detected with ehemiluminescent immunoassay, the proportion of AFP L3 levels were calculated, and the relationship between the elevated AFP-L3 （ % ） levels and benign and malignant liver disease was analyzed. Results There were significant differences in positive rate between the patients of HCC, suspected HCC and other liver disease （81.80%, 73.68%, 11.80%, respectively, P 〈 0.05）. Among the undetermined HCC （suspected HCC, liver disease） patients, 12 out of 21 cases of AFP-13 positive were diagnosed to be HCC within 6 months, and 6 of them were diagnosed to be the single small HCC at the early stage through B-Ultrasonic Diagnosis or CT. Among 62 cases of AFP-L3 negative, 3 cases were diagnosed to be HCC within 6 months and the risk of occurrence of HCC for AFP-L3 positive increased l 1.9 times. Conclusion AFP-L3 has no correlation with AFP value, and it can be used as an independent HCC diagnosis factor. The detection of AFP-L3 has a significant implication for the identification of benign or malignant liver disease and the early stage predictive diagnosis of HCC while AFP increases.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2008年第5期336-338,共3页
Chinese Journal of Experimental and Clinical Virology
基金
北京市首都医学发展基金重点支持项目（2005-2038）
关键词
甲胎蛋白类
癌
肝细胞
Alpha-fetoproteins
Carcinoma, hepatocellular
