摘要
目的:通过不同途径移植羊膜间充质干细胞治疗脑损伤大鼠,比较脑损伤大鼠行为学的改善,为脑损伤治疗选择有效的移植途径。方法:制作80只Wistar大鼠脑损伤模型,分为对照组与治疗组。对照组为单纯脑损伤大鼠,共20只;治疗组分为尾静脉移植组(A组)、侧脑室移植组(B组)、脑损伤区移植组(C组),每组20只,均给与植入人羊膜间充质干细胞,采用NSS评分法评定治疗效果。结果:脑损伤区移植组大鼠行为学改善与其他各组相比,差异有统计学意义(P<0.05);侧脑室移植组分别与尾静脉移植组及对照组相比,差异有统计学意义(P<0.05);而尾静脉移植组与对照组相比,差异无统计学意义(P>0.05)。结论:脑损伤区移植羊膜间充质干细胞是治疗脑损伤大鼠的有效途径。
Objective To compare the behavioral improvement to find the best transplantation approach for treating brain injury through transplanting amniotic-derived mesenehymal stem cells into brain injured rats in different ways. Methods Eighty brain injured Wista rats were randomly divided into a control group with brain injury alone （n = 20） and a treatment group （n = 60） which were further evenly divided into Group A （ transplanted through the vena caudalis） , Group B （ transplanted through the ventriculus cerebri lateralis ）, and Group C （transplanted through the injured brain area）. Each group was transplanted with amniotic-derived esenchymal stem cells, and their therapeutic efficacy would be evaluated through the neurological severity score （NSS）. Results Compared with other groups, the behaviors of Group C had markedly improved. There was statistically significant difference in the 2 groups （P 〈0.05 ）. Compared with the control group, the behaviors of Group A and Group B had marked improvement. There was statistically significant difference in the 3 groups （P 〈0.05）. However, there was no significant difference between Group A and the control group （P 〉0.05）. Conelusion Transplanting the amniotic-derived mesenchymal stem cells into the injured brain area may be effective for brain injury in rats.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2008年第10期926-930,共5页
Journal of Central South University ：Medical Science
基金
河南省医学科技创新人才工程项目(2005018)~~
关键词
羊膜间充质干细胞
移植
大鼠
脑损伤
amniotic-derived mesenchymal
injury stem cell
transplantation
rats
brain
