摘要
建立用高效液相色谱-二极管阵列法测定生鲜乳及乳制品中的三聚氰胺的检测方法。本实验参照FDA三聚氰胺检测方法、NY/T1372—2007,探讨检测三聚氰胺的色谱条件和优化生鲜乳及乳制品中的三聚氰胺前处理方法。最后确立了有效的检测方法,采用硫酸铵(0.02mol/L):甲醇=94:6(V/V)作为流动相,203nm为检测波长。样品溶液在0.1～150μg/ml之间均具有良好的线性关系,最低检测限为0.5mg/kg,回收率均在91%以上,回归方程式为y=2.37×105x+1.21×104及相关系数r=0.9998。此法操作简便、快速、灵敏、准确,样品处理简便易行,适于测定生鲜乳及乳制品中的三聚氰胺。
A high performance liquid chromatography- photodiode array detector for determining the content of melamine in fresh milk and dairy produce was studied. Referring to FDA method and NY/T 1372- 2007 of determination melamine, the chromatogram conditions for determining the content of melamine were discussed and the predisposal method of melamine in fresh milk and dairy product was optimized. The optimal detection conditions are determined as follows： 0. 02mol/L ammonium sulfate-methanol （94： 6, V/V） as mobile phase, and detection wavelength 203 nm. The good linearity is in the range of from 0.1 to 150 u g/ml, the detection limit is 0.5 mg/kg, the average recoveries of melamine are above 91%, and the regression equation is y=2.37 × 10^5x＋1.21 × 10^4 with the coefficient 0.9998. The method is simple, rapid, sensitive and exact with convenient sample treatment method feasible. It is suitable for the determination of melamine in fresh milk and dairy product.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第10期531-535,共5页
Food Science
关键词
高效液相色谱
二极管阵列检测器
生鲜乳
乳制品
三聚氰胺
high performance liquid chromatography
photodiode array detector
fresh milk
dairy product
melamine
