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不同砧木嫁接对NaCl胁迫下黄瓜幼苗生长、渗透调节和酶活性的影响 被引量:5

Effects of Grafting with Different Rootstocks on the Growth,Osmotic Adjustment and Anti-oxidant Enzyme Activities of Cucumber Seedlings under Salt Stress
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摘要 采用‘超丰8848'和‘黑籽南瓜'作砧木,‘津春2号'黄瓜作接穗嫁接,以‘津春2号'自根苗为对照,研究了NaCl胁迫对不同砧木嫁接黄瓜苗生长及生理特性的影响。结果表明,NaCl胁迫下,嫁接苗地上部和根干重、叶面积、茎粗受抑制的程度显著低于自根苗;嫁接苗叶片中MDA、H_2O_2含量、超氧阴离子产生速率显著低于自根苗,而叶片中可溶性糖含量、地上部含水量、SOD、POD、CAT、APX活性显著高于自根苗。NaCl胁迫下,‘超丰8848'嫁接苗生长受抑制程度、叶片中MDA、H_2O_2含量显著低于‘黑籽南瓜'嫁接苗,脯氨酸含量、可溶性蛋白质含量、地上部含水量、SOD、POD、CAT、APX、GR活性显著高于‘黑籽南瓜'嫁接苗。具有更好的渗透调节和抗氧化能力是‘超丰8848'嫁接苗耐盐性高于‘黑籽南瓜'嫁接苗的重要原因。 The effects of grafting with different rootstocks on cucumber growth and physiological trait were investigated by using 'Chaofeng 8848' and 'Heizinangua' as rootstocks, 'Jinchun No.2' as scion under NaCl stress, and the ungrafted cucumber plants were as the controls. The results showed that the reductions in the shoot and root dry weights, leaf area, stem diameter of grafted plants were lower than those of ungrafted plants under the same NaCl stress. MDA and H2O2 contents, O2^- producing rate were lower, whereas soluble sugar, shoot water content, SOD, POD, CAT, APX activities in the leaves of grafted plants were higher than those of ungrafted plants at the same NaCl stress. The reductions in shoot and root dry weight, leaf area and stem diameter of grafted 'Chaofeng 8848' plants were significantly lower than those of grafted 'Heizinangua' plants ones. MDA and H2O2 contents were lower whereas proline contents, soluble protein content, SOD, POD, CAT, APX, GR activities in the leaves of grafted 'Chaofeng 8848' plants were higher than those of grafted 'Heizinangua' plants under the same NaCL stress. The better osmotic adjustment ability and antioxidant system was the important reason for the higher salt tolerance of grafted ' Chaofeng 8848' plants than grafted ' Heizinangua' ones.
出处 《上海交通大学学报(农业科学版)》 2008年第5期393-397,共5页 Journal of Shanghai Jiaotong University(Agricultural Science)
基金 国家科技支撑计划(2006BAD07B04) 湖北省科技攻关(2003AA201C30) 武汉市科技攻关项目(20062002040)资助
关键词 嫁接 NACL 黄瓜 生长 生理特性 grafting NaCl, Cucumis sativus L. growth physiological trait
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