摘要
目的:探讨碳酸酐酶Ⅸ(carbonic anhydrases Ⅸ,CAⅨ)和血管内皮生长因子(vascular endothelial growth factor,VEGF)在非小细胞肺癌(NSCLC)组织中的表达及与临床病理参数的关系。方法:应用免疫组化方法检测84例NSCLC和20例正常肺组织中CAⅨ和VEGF的表达。结果:NSCLC组织中CAⅨ和VEGF阳性表达率分别为71.4%(60/84)和66.7%(56/84),均显著高于正常肺组织(0),χ2=9.33,χ2=7.29,P<0.01。CAⅨ的表达与病理组织类型、肿瘤大小、临床分期及淋巴结转移相关。鳞癌患者的CAⅨ阳性率明显高于腺癌患者,χ2=8.36,P<0.05;且随临床分期Ⅰ～Ⅳ期的顺序明显上升,P<0.05。有淋巴结转移组CAⅨ阳性表达率明显高于无淋巴结转移组,P<0.01。VEGF的表达与肿瘤的大小、淋巴结转移及临床分期相关,有淋巴结转移组VEGF阳性表达率[80.8%(42/52)]显著高于无淋巴结转移组[43.8%(14/32)],χ2=10.75,P<0.01;Ⅰ～Ⅱ期VEGF阳性表达率[55.8%(24/43)]明显低于Ⅲ～Ⅳ期[78.0%(32/41)],χ2=9.00,P<0.05。CAⅨ和VEGF的表达呈正相关,r=0.405,P<0.01。结论:CAⅨ和VEGF高表达均与NSCLC的发生、发展和浸润转移有关,两者可能起协同作用。
OBJECTIVE：To explore the expressions and role of carbonic anhydrases Ⅸ （CAⅨ） and vascular endothelial growth factor （VEGF） in non-small cell lung cancer. METHODS： The expressions of CAⅨ and VEGF in 86 pathologically diagnosed non-small cell lung cancer and normal lung tissues were detected by immunohistochemical technique. Results：The overall positive expression rates of CAⅨ and VEGF in NSCLC were 71.4% and 66.7%, repectively, which were both higher than that in normal lung tissue, P〈0.01. The expression of CAⅨ was related to histfologic type, tumor size, clinical stage and status of lymphatic wode. CAⅨ expression positive rate in cases with squamous cancer was higher than that in adenocarcinoma, P〈0.05, and the positive rate increased with the progression of the clinical stage from Ⅰ to Ⅳ. CAⅨ expression in patients with lymph node involvement was higher than that in lymph node negative, there was statistically significant difference, P〈0.01. The expression of VEGF was related to tumor size, clinical stage and status of lymphatic node. VEGF expression in patients with lymph node involvement[80.8%（42/52）] was significantly higher than that in lymph node negative[43.8%（14/32）], χ^2=10.75, P〈0.01. The VEGF positive rate in cases with stage Ⅰ and Ⅱ [55.8%（24/43）]was markedly lower than that in stage Ⅲ and Ⅳ[78.0%（32/41）], χ^2=9.00 P〈0.05. There was a positive association between CAⅨ and VEGF, r=0.405, P〈0.01. CONCLUSIONS： The hypoxia and angiogenesis are important markers of carcinoma. CAⅨ and VEGF are involved in the tumorgenesis and progression of non-small cell lung cancer. CAⅨ and VEGF might play synergetic roles in invasion and metastasis of the disease.
出处
《中华肿瘤防治杂志》
CAS
2008年第16期1251-1254,共4页
Chinese Journal of Cancer Prevention and Treatment
关键词
癌
非小细胞肺/病理学
血管内皮生长因子
免疫组织化学
carcinoma, non-small cell lung/pathology, vascular endothelial growth factor, immunohistochemistry
