摘要
目的:观察一氧化氮(NO)与转化生长因子-β1(TGF-β1)在放射性肺损伤形成过程中的协同作用。方法:实验兔分为对照组和L-NAME组,采用6MVX线对左全肺进行照射,25Gy,1次。将2组动物再各自随机分为3组,分别于照射后1、3和6个月各自处死一组动物,照射前及处死前进行双肺血流同位素扫描及左肺肺泡灌洗。进行灌洗液NO及TGF-β1测定及肺组织病理分期。结果:在照射后1个月,对照组及L-NAME组的NO含量分别为(0.48±0.26)和(0.30±0.11)μmol/L,均明显高于放疗前水平,L-NAME组的升高幅度低于对照组。在照射后1、3和6个月,对照组的TGF-β1含量分别为(0.58±0.15)、(0.30±0.10)和(0.59±0.28)ng/mL,均明显高于放疗前水平,L-NAME组TGF-β1的升高幅度低于对照组。放疗后肺血流呈持续下降趋势。对照组放射性肺炎发生率(5/5)高于L-NAME组(2/5)。结论:NO在放疗早期的明显升高放疗后肺血流的持续下降是放射性肺损伤的发病机制之一;NO与TGF-β1在放射性肺损伤的形成过程中具有协同作用。
OBJECTIVE：To observe the cooperation of NO and TGF-β1 on the formation of radiation pneumonitis （RP）. METHODS： The experimental rabbits were assigned to the control group and L-NAME group. All the left lung of the rabbits were irradiated with 6 MV X-rays, 25 Gy in one fraction. The rabbits in each of the two groups were assigned randomly to three sub-groups and executed at the first, third and 6th month after the radiation. The blood-flux isotope scanning and the flushing in left lung were done before the radiation and after the execution. NO and TGF-β1 were measured and the pathomorphological changes were evaluated. RESUTLTS： The contents of NO in the two groups（L-NAME： 0.30±0.11 μmol/L, control： 0.48±0.26 μmol/L） increased remarkably at the first month after the radiation, and the L-NAME group was lower than the control in range. The contents of TGF-β1 in the two groups increased remarkably at the 1st[（0.58±0.15） ng/mL], 3rd[（0.30±0.10） ng/mL], 6th[（0.59±0.28） ng/mL] month after the radiation, and the L-NAME group was lower than the control in range. Lung blood-flux kept the decreasing tread after the radiation. The control group （5/5） was higher than the L-NAME group （2/5） at ratio of radiation pneumonitis. CONCLUSIONS： That NO increases remarkably at the first month after the radiation and lung blood-flux kept the decreasing tread after the radiation is one of the key of the formation of radiation pneumonitis. NO is cooperated with TGF-β1 on the formation of radiation pneumonitis.
出处
《中华肿瘤防治杂志》
CAS
2008年第16期1230-1232,共3页
Chinese Journal of Cancer Prevention and Treatment
关键词
辐射性肺炎
放射疗法/副作用
一氧化氮
转化生长因子Β
radiation pneumonitis, radiotherapy/side effects, nitric oxide, transforming growth factor beta
