摘要
目的建立实验红鲫C1HD系生化遗传标记。方法采用聚丙烯酰胺梯度凝胶垂直电泳法,分析实验红鲫C1HD系与普通红鲫的苹果酸脱氢酶(MDH)和超氧化物歧化酶(SOD)等同工酶。结果实验红鲫C1HD系和普通红鲫血清蛋白电泳可分离出25条以上蛋白带,分为A、B、C等3个区段。在A、B区段,实验红鲫C1HD系分别具有SP-A1谱带和SP-B1、SP-B3~8谱带,但缺少SP-A2、SP-A3和SP-B2谱带。实验红鲫C1HD系具有8条SOD同工酶电泳谱带,比普通红鲫多出SOD-3、SOD-8两条特征带。两种红鲫均具备MDH-1、MDH-2和MDH-3电泳谱带,且带型一致;普通红鲫额外具备MDH-4和MDH-5两条电泳谱带。结论血清蛋白SP-A1和超氧化物歧化酶SOD-3、SOD-8电泳谱带可以作为实验红鲫C1HD系的生化遗传标记。
Objective To set up biochemical genetic markers of the inbred strain C1HD of Carrassius aurratus red variety as a laboratory animal. Methods MDH, SOD and serum proteins of both the inbred strain C1HD of Carassius auratus red variety and the wild ones were analyzed by polyacrylamide gradient gel vertical electrophoresis. Results More than twenty-five bands of serum proteins from the two types of Carassius auratus red variety were separated, which could be divided into A, B and C regions. There were 14 bands at the region C near the anode, while an apparent SP-A1 band at region A near the cathode was detected in the inbred strain C1HD of carassius auratus red variety, but SP-A2 and SP-A3 were not detected. There were 8 bands at the region B, while SP-B2 was not detected in the inbred strain C1HD of Carassius auratus red variety. There were three MDH isozyme electrophoretic bands of inbred strain C1HD of Carassius auratus red variety （MDH-1, MDH-2, MDH-3） and those were five （MDH-1, MDH-2, MDH-3, MDH^4, MDH-5） in the wild ones. There were eight SOD isozyme electrophoretic bands of the inbred strain C1HD of Carassius auratus red variety and six in the wild ones, and the former have two more bands （SOD-3, SOD- 8） than the latter, but the respective of the two fishes are the same. Conclusion SP-A1, SOD-3 and SOD-8 of electrophoretic bands can be used as biochemical genetic markers of the inbred strain C1HD of Carassius auratus red variety.
出处
《中国实验动物学报》
CAS
CSCD
2008年第5期357-360,共4页
Acta Laboratorium Animalis Scientia Sinica
关键词
红鲫
C1HD系
血清蛋白
同工酶
生化遗传标记
Carassius auratus red variety
Inbred strain C1HD
Serum protein
Isozyme
Biochemical genetic marker
